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Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1844-1852.doi: 10.16420/j.issn.0513-353x.2023-0555

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles     Next Articles

Molecular Characterization of the Odontoglossum Ringspot Virus and Cymbidium Chlorotic Mosaic Virus Mix-Infecting Cymbidium hybridum in Yunnan

JIN Xiaoxiang1, YAO Junxi1, YIN Yueyan3, LI Tingting2, GUO Miao3, ZHONG Jing2, HE Shouxing3, CHEN Yue2, HE Jiawei3, YAN Bo1,**(), DING Ming2,**()   

  1. 1 Department of Landscape and Horticulture,Southwest Forestry University,Kunming 650224,China
    2 Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650223,China
    3 Institute of Alpine Economic Plants,Yunnan Academy of Agricultural Sciences,Lijiang,Yunnan 674100,China
  • Received:2023-09-14 Revised:2024-05-17 Online:2024-08-25 Published:2024-08-21
  • Contact: YAN Bo, DING Ming

Abstract:

Forty greenhouse-grown orchid samples were collected in Lijiang City,Yunnan Province,China,to assess the presence of viruses. Total RNAs from two samples were extracted and subjected them to high-throughput transcriptome sequencing(HTS)using the Illumina HiSeq platform. BLASTN searches revealed the presence of contigs with high similarities to cymbidium mosaic virus(CymMV),odontoglossum ringspot virus(ORSV),and cymbidium chlorotic mosaic virus(CyCMV). RT-PCR assays were conducted to confirm the presence of these viruses in the individual collected samples,and 10 samples were found to be mix-infected by ORSV and CyCMV. To further ascertain their molecular characteristics,the complete genomic sequences of the ORSV-DH and CyCMV-DH isolates were cloned. The full-length sequences of the ORSV-DH and CyCMV-DH genomes were determined to be 6 611 nt (GeneBank accession No. OP644547)and 4 083 nt(GeneBank accession No. OP629812),respectively. Sequence alignments revealed that ORSV-DH displayed nucleotide sequence identities of 97.69%- 99.41% with previously reported ORSV isolates,whereas CyCMV-DH showed identities of 89.84%- 98.19% with other CyCMV isolates. Phylogenetic trees of ORSV-DH and CyCMV-DH based on their complete genomic sequences were constructed and showed closely related to the Japanese isolate ORSV-Cy-1(S83257)and the Japanese isolate CyCMV-Japan(NC_027123),respectively.

Key words: Cymbidium hybridum, ORSV, CyCMV, characterization of genomic sequence, mix-infection