Genetic Diversity of Chinese Herbaceous Peony Resources from Various Cultivation Areas Based on SSR Markers

doi:10.16420/j.issn.0513-353x.2021-1220

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (4): 875-884.doi: 10.16420/j.issn.0513-353x.2021-1220

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Genetic Diversity of Chinese Herbaceous Peony Resources from Various Cultivation Areas Based on SSR Markers

WAN Yingling¹, JIN Yijia¹, LIU Aiqing², ZHU Mengting¹, LIU Yan¹^,^*()

1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding，National Engineering Research Center for Floriculture，Beijing Laboratory of Urban and Rural Ecological Environment，School of Landscape Architecture，Beijing Forestry University，Beijing 100083，China
2. Urban Management Development Center of Heze，Heze，Shandong 274000，China

Received:2022-11-22 Revised:2023-02-26 Online:2023-04-25 Published:2023-04-27
Contact: *（E-mail：chblyan@163.com） E-mail:chblyan@163.com

Abstract

Abstract:

Genetic diversity of 386 ornamental herbaceous peonies from four cultivation areas were estimated based on genetic variation and genetic distance by amplification of 33 pairs of SSR fluorescent primers. The results showed that (1) There was rich genetic diversity in Chinese ornamental herbaceous peonies. The polymorphism information content（PIC）of primers was 0.62-0.84，average observation heterozygosity（H_o）and expected heterozygosity（H_e）was 0.73 and 0.78，respectively，and Shannon information index（I）was 1.35-2.08. (2) The genetic diversity of varieties from various cultivation areas in descending order was Heze，Luoyang，Yangzhou and Lintao. (3) AMOVA analysis indicated the variance mainly came from within cultivation areas, genetic differentiation coefficient（F_ST）among cultivation areas was 0.031，and the gene flow（N_m）was 7.795. (4) Genetic distances among the varieties in each cultivated areas ranged from 0.032 to 0.123，and the genetic consistency ranged from 0.884 to 0.969. The results of principal coordinate analysis and clustering revealed that genetic differentiation was not related to the cultivated areas. It can be concluded that there was abundant genetic information in Chinese ornamental herbaceous peonies. Cultivars from four cultivation areas did not show obvious genetic differentiation，but existed frequent exchanges.

Key words: Paeonia lactiflora group, genetic differentiation, cultivation source, clustering

CLC Number:

S682.12

WAN Yingling, JIN Yijia, LIU Aiqing, ZHU Mengting, LIU Yan. Genetic Diversity of Chinese Herbaceous Peony Resources from Various Cultivation Areas Based on SSR Markers[J]. Acta Horticulturae Sinica, 2023, 50(4): 875-884.

Figures/Tables 7

References 36

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位点 Locus	重复基序 Repeat motif	产物大小/bp Size range	等位基因数 Number of observed alleles （N_a）	有效等位基因数 Number of effective alleles （N_e）	香农信息指数 Shannon information index （I）	观测杂合度 Observation heterozygosity （H_o）	期望杂合度 Expected heterozy- gosity（H_e）	固定系数 Fixed index （F）	多态信息含量 Polymorphism information content （PIC）
T852	(AT)8	158 ~ 367	26	3.99	1.82	0.40	0.75	0.46	0.72
S024	(AAT)16	161 ~ 201	23	4.32	1.92	0.72	0.77	0.07	0.74
TA704	(TC)17	237 ~ 277	22	4.62	1.95	0.51	0.78	0.35	0.76
TA564	(TA)8	172 ~ 201	21	5.00	1.97	0.77	0.80	0.04	0.78
S025	(ATT)7	268 ~ 350	19	5.36	2.01	0.68	0.81	0.17	0.79
TA038	(CT)9	259 ~ 299	19	5.20	2.05	0.23	0.81	0.72	0.79
TA133	(AT)8	258 ~ 286	17	4.46	1.88	0.56	0.78	0.28	0.75
T300	(AT)10	218 ~ 238	17	3.20	1.62	0.55	0.69	0.20	0.66
T125	(TC)9	128 ~ 170	15	4.79	1.78	0.86	0.79	-0.08	0.76
T356	(AG)9	247 ~ 269	15	4.18	1.70	0.34	0.76	0.56	0.72
TA700	(AG)9	278 ~ 312	15	4.99	1.90	0.81	0.80	-0.01	0.77
T205	(TC)9	275 ~ 301	14	5.80	2.04	0.83	0.83	0.00	0.81
TA028	(AC)6	101 ~ 123	14	3.54	1.63	0.74	0.72	-0.03	0.69
TA134	(GA)16	142 ~ 224	14	4.60	1.92	0.90	0.78	-0.16	0.76
S237	(CTG)8	193 ~ 219	14	4.53	1.78	0.84	0.78	-0.08	0.75
T160	(CT)10	205 ~ 227	13	6.91	2.08	0.82	0.86	0.04	0.84
TA464	(TC)8	200 ~ 214	13	6.23	1.95	0.80	0.84	0.05	0.82
F106	(TATG)5	200 ~ 290	13	5.31	1.84	0.76	0.81	0.06	0.79
SA010	(AAT)5	264 ~ 294	13	3.91	1.66	0.73	0.74	0.01	0.71
TA673	(CA)18	190 ~ 288	12	3.64	1.54	0.90	0.72	-0.24	0.68
T241	(GA)15	127 ~ 157	12	5.75	1.95	0.90	0.83	-0.08	0.81
TA074	(CT)8	260 ~ 306	12	4.22	1.73	0.89	0.76	-0.17	0.73
TA082	(AG)6	242 ~ 280	11	5.03	1.74	0.72	0.80	0.10	0.77
TA610	(TC)8	257 ~ 291	11	4.42	1.67	0.79	0.77	-0.02	0.74
TA086	(AG)10	393 ~ 419	10	3.04	1.39	0.63	0.67	0.05	0.62
T317	(CT)10	157 ~ 183	10	5.94	1.89	0.92	0.83	-0.11	0.81
T237	(CT)10	95 ~ 109	10	4.24	1.65	0.73	0.76	0.04	0.73
T040	(GA)7	228 ~ 246	9	4.53	1.66	0.71	0.78	0.09	0.75
T179	(AT)7	235 ~ 269	9	3.88	1.46	0.68	0.74	0.09	0.70
T863	(AG)10	140 ~ 160	9	4.37	1.70	0.82	0.77	-0.06	0.74
S853	(TTG)5	158 ~ 186	9	5.15	1.76	0.83	0.81	-0.03	0.78
T304	(GA)10	147 ~ 163	8	4.45	1.67	0.87	0.78	-0.12	0.74
T163	(TA)8	146 ~ 160	7	2.99	1.35	0.70	0.67	-0.05	0.62
均值Mean			13.82	4.62	1.78	0.73	0.78	0.06	0.75
标准误SE			0.80	0.16	0.03	0.03	0.01	0.04	0.01

位点 Locus	重复基序 Repeat motif	产物大小/bp Size range	等位基因数 Number of observed alleles （N_a）	有效等位基因数 Number of effective alleles （N_e）	香农信息指数 Shannon information index （I）	观测杂合度 Observation heterozygosity （H_o）	期望杂合度 Expected heterozy- gosity（H_e）	固定系数 Fixed index （F）	多态信息含量 Polymorphism information content （PIC）
T852	(AT)8	158 ~ 367	26	3.99	1.82	0.40	0.75	0.46	0.72
S024	(AAT)16	161 ~ 201	23	4.32	1.92	0.72	0.77	0.07	0.74
TA704	(TC)17	237 ~ 277	22	4.62	1.95	0.51	0.78	0.35	0.76
TA564	(TA)8	172 ~ 201	21	5.00	1.97	0.77	0.80	0.04	0.78
S025	(ATT)7	268 ~ 350	19	5.36	2.01	0.68	0.81	0.17	0.79
TA038	(CT)9	259 ~ 299	19	5.20	2.05	0.23	0.81	0.72	0.79
TA133	(AT)8	258 ~ 286	17	4.46	1.88	0.56	0.78	0.28	0.75
T300	(AT)10	218 ~ 238	17	3.20	1.62	0.55	0.69	0.20	0.66
T125	(TC)9	128 ~ 170	15	4.79	1.78	0.86	0.79	-0.08	0.76
T356	(AG)9	247 ~ 269	15	4.18	1.70	0.34	0.76	0.56	0.72
TA700	(AG)9	278 ~ 312	15	4.99	1.90	0.81	0.80	-0.01	0.77
T205	(TC)9	275 ~ 301	14	5.80	2.04	0.83	0.83	0.00	0.81
TA028	(AC)6	101 ~ 123	14	3.54	1.63	0.74	0.72	-0.03	0.69
TA134	(GA)16	142 ~ 224	14	4.60	1.92	0.90	0.78	-0.16	0.76
S237	(CTG)8	193 ~ 219	14	4.53	1.78	0.84	0.78	-0.08	0.75
T160	(CT)10	205 ~ 227	13	6.91	2.08	0.82	0.86	0.04	0.84
TA464	(TC)8	200 ~ 214	13	6.23	1.95	0.80	0.84	0.05	0.82
F106	(TATG)5	200 ~ 290	13	5.31	1.84	0.76	0.81	0.06	0.79
SA010	(AAT)5	264 ~ 294	13	3.91	1.66	0.73	0.74	0.01	0.71
TA673	(CA)18	190 ~ 288	12	3.64	1.54	0.90	0.72	-0.24	0.68
T241	(GA)15	127 ~ 157	12	5.75	1.95	0.90	0.83	-0.08	0.81
TA074	(CT)8	260 ~ 306	12	4.22	1.73	0.89	0.76	-0.17	0.73
TA082	(AG)6	242 ~ 280	11	5.03	1.74	0.72	0.80	0.10	0.77
TA610	(TC)8	257 ~ 291	11	4.42	1.67	0.79	0.77	-0.02	0.74
TA086	(AG)10	393 ~ 419	10	3.04	1.39	0.63	0.67	0.05	0.62
T317	(CT)10	157 ~ 183	10	5.94	1.89	0.92	0.83	-0.11	0.81
T237	(CT)10	95 ~ 109	10	4.24	1.65	0.73	0.76	0.04	0.73
T040	(GA)7	228 ~ 246	9	4.53	1.66	0.71	0.78	0.09	0.75
T179	(AT)7	235 ~ 269	9	3.88	1.46	0.68	0.74	0.09	0.70
T863	(AG)10	140 ~ 160	9	4.37	1.70	0.82	0.77	-0.06	0.74
S853	(TTG)5	158 ~ 186	9	5.15	1.76	0.83	0.81	-0.03	0.78
T304	(GA)10	147 ~ 163	8	4.45	1.67	0.87	0.78	-0.12	0.74
T163	(TA)8	146 ~ 160	7	2.99	1.35	0.70	0.67	-0.05	0.62
均值Mean			13.82	4.62	1.78	0.73	0.78	0.06	0.75
标准误SE			0.80	0.16	0.03	0.03	0.01	0.04	0.01

抽样 Sampling	样本数 Numbers of sampling （N）	等位基因数 Number of observed alleles （N_a）	有效等位基因数 Number of effective alleles （N_e）	香农信息指数 Shannon information index （I）	观测杂合度 Observation heterozygosity （H_o）	期望杂合度 Expected heterozygosity（H_e）
第1次1st	29.75 ± 0.04	6.91 ± 0.15	3.98 ± 0.10	1.53 ± 0.02	0.71 ± 0.02	0.73 ± 0.01
第2次2nd	29.75 ± 0.04	7.30 ± 0.15	4.04 ± 0.09	1.57 ± 0.02	0.72 ± 0.02	0.73 ± 0.01
第3次3rd	29.75 ± 0.04	7.32 ± 0.15	4.12 ± 0.10	1.58 ± 0.02	0.71 ± 0.02	0.74 ± 0.01
全部Total	96.49 ± 4.27	9.13 ± 0.26	4.18 ± 0.10	1.62 ± 0.02	0.72 ± 0.02	0.74 ± 0.01

抽样 Sampling	样本数 Numbers of sampling （N）	等位基因数 Number of observed alleles （N_a）	有效等位基因数 Number of effective alleles （N_e）	香农信息指数 Shannon information index （I）	观测杂合度 Observation heterozygosity （H_o）	期望杂合度 Expected heterozygosity（H_e）
第1次1st	29.75 ± 0.04	6.91 ± 0.15	3.98 ± 0.10	1.53 ± 0.02	0.71 ± 0.02	0.73 ± 0.01
第2次2nd	29.75 ± 0.04	7.30 ± 0.15	4.04 ± 0.09	1.57 ± 0.02	0.72 ± 0.02	0.73 ± 0.01
第3次3rd	29.75 ± 0.04	7.32 ± 0.15	4.12 ± 0.10	1.58 ± 0.02	0.71 ± 0.02	0.74 ± 0.01
全部Total	96.49 ± 4.27	9.13 ± 0.26	4.18 ± 0.10	1.62 ± 0.02	0.72 ± 0.02	0.74 ± 0.01

栽培地 Culture place	样本数 Numbers of sampling （N）	等位基因数 Number of observed alleles （N_a）	有效等位基因数 Number of effective alleles （N_e）	观测杂合度 Observation heterozygosity （H_o）	期望杂合度 Expected heterozygosity （H_e）	香农信息指数 Shannon information index（I）	遗传分化系数 Genetic differentiation index（F_ST）	基因流 Gene flow （N_m）
菏泽Heze	165	10.79	4.57	0.75	0.77	1.74
洛阳Luoyang	93	9.67	4.35	0.74	0.76	1.68
扬州Yangzhou	97	9.48	4.14	0.68	0.74	1.62
临洮Lintao	29	6.61	3.66	0.69	0.69	1.45
全部Total							0.031	7.795

Genetic Diversity of Chinese Herbaceous Peony Resources from Various Cultivation Areas Based on SSR Markers

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变异来源 Source of variation	自由度 df	方差总和 SS	平均方差 MS	变异组分 Est. Var.	变异百分率/% Variation
栽培地间Among cultivation areas	3	504.584	168.195	1.609	6
栽培地内Within cultivation areas	382	10 017.584	26.224	26.224	94
总和Total	385	10 522.168		27.833	100

栽培地 Culture place	菏泽 Heze	洛阳 Luoyang	扬州 Yangzhou	临洮 Lintao
菏泽Heze		0.931	0.969	0.957
洛阳Luoyang	0.072		0.950	0.884
扬州Yangzhou	0.032	0.051		0.933
临洮Lintao	0.044	0.123	0.069

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