Abstract: Pineapple mealybug wilt associated virus-2（PMWaV-2）is an important pathogen that causes Mealybug wilt of pineapple（MWP）. This study established a real-time quantitative RT-PCR method with TaqMan probe based on specific primers of conserved nucleotide sequence of PMWaV-2 coat protein gene. The results showed that the method is highly sensitive to positive sample，but has no fluorescence signal to health sample and water control. The sensitivity of real-time quantitative RT-PCR is about 100 times higher than regular PCR. Three-time repeats revealed that the coefficients of variation between the intra- and inter-assay were both within 1.85%，indicating a simple，specificity，high sensitivity，and reliable reproducibility detection method to PMWaV-2.

Key words: pineapple, PMWaV-2, TaqMan probe, real-time quantitative RT-PCR, detction