Development of SCAR Marker Identifying the Cytoplasmic Male Sterility Gene in Bunching Onion（Allium fistulosum L.）

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (7): 1382-1388.

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Development of SCAR Marker Identifying the Cytoplasmic Male Sterility Gene in Bunching Onion（Allium fistulosum L.）

GAO Li-min，DONG Fei，HUO Yu-meng，LIU Bing-jiang，MIAO Jun，and CHEN Yun-qi^*

Vegetable Research Institute of Shandong Academy of Agricultural Sciences，Key Laboratory for Biology of Greenhouse，Vegetable of Shandong Province，National Improvement Center for Vegetable，Shandong Branch，Jinan 250100，China

Received:2013-02-06 Online:2013-07-25 Published:2013-07-25

Abstract

Abstract: Cytoplasmic genetic male-sterility（CMS）is used to produce hybrid bunching onion（Allium fistulosum L.）seeds worldwide. In this paper，we developed one SCAR marker using male sterile line（980238A）and maintainer line （980238B）as sterile and fertile gene pool for the target band screening.This marker was 607 bp in size and designated as S607 which was specifically amplified in the male sterile line，and it could distinguish two cytoplasm types CMS-S（Sterile type）and CMS-N（Normal type）. The SCAR marker was verified in five male sterile lines，five maintainer lines，and four cross combinations，all of these valuations were in accord with their cytoplasmic type（S or N）. The result of this study indicates that the SCAR markers of S607 will greatly contribute to establish a molecular marker assisted breeding program in bunching onion.

Key words: bunching onion, cytoplasm, male sterility, SCAR marker

CLC Number:

S 633.1

GAO Li-Min, DONG Fei, HUO Yu-Meng, LIU Bing-Jiang, MIAO Jun, CHEN Yun-Qi. Development of SCAR Marker Identifying the Cytoplasmic Male Sterility Gene in Bunching Onion（Allium fistulosum L.）[J]. ACTA HORTICULTURAE SINICA, 2013, 40(7): 1382-1388.

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Development of SCAR Marker Identifying the Cytoplasmic Male Sterility Gene in Bunching Onion（Allium fistulosum L.）

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