Abstract: The flower buds of garlic（Allium sativum L.） were used to get well-developed floral organ by means of garlic stem culture in vitro. With the unfertilized ovary，a regeneration system of garlic in vitro was established by analysis the effects of different medium（MS，B5，N6）and different hormones on haploid induction. The suitable media for callus induction was B5 + 2 mg · L^-1 6-BA + 1 mg · L^-1 NAA，the highest induction rate of callus and gynogenic embryos was 12.24%；The suitable media for adventitious bud was B5 + 3 mg · L^-1 6-BA + 1 mg · L^-1 NAA，the regeneration frequencies was 46.15%；Adventitious bud and embryos could grow root and develop into complete plant in the media B5 + 0.05 mg · L^-1 NAA. Eighteen regeneration plants were obtained in total. Ploidy analysis of the regenerations with flow cytometry analysis of leaf tissue revealed that 16 regeneration palnts are haploid.

Key words: garlic, haploid, unfertilized ovary, flow cytometry