Abstract: The investigation of differentially expressed proteins of red cultivar‘Dayemei’and white
cultivar‘Shuijing’of Myrica rubra in ripening time were conducted by using 2-DE coupled with
MALDI-TOF-TOF/MS approaches. The comparison of the protein patterns of‘Dayemei’and ‘Shuijing’
showed 41 differential expressed protein spots in these two cultivars，of which 33 were identified，among
them，29 proteins up-regulated，3 proteins specifically expressed and 1 down-regulated in‘Dayemei’. The
identified proteins were classified according to their functions which involved in anthocyanin metabolism（12%），carbohydrate and energy metabolism（30%），protein metabolism（18%）
and defense response（15%）. We found that the enzymes of anthocyanidin synthase（ANS），phenylalanine
amonnia lyase（PAL），chalcone synthase 11（CHS11）and UDP-glucose︰flavonoid 3-O-glucosyltransferase
（ UFGT ） involved in anthocyanin synthesis were successfully identified. These enzymes were
up-regulated obviously in‘Dayemei’. Meanwhile，9 proteins involved carbohydrate metabolism were also
up regulated. Up-regulated the key proteins involved in anthocyanin and carbohydrate metabolism were
important for anthocyanin accumulation and color formation in Myrica rubra.

Key words: Myrica rubra, anthocyanins, carbohydrate metabolism, differentially expressed proteins