Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (3): 588-592.

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Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH

REN Gui-Ling, ZHANG Xian-Xian, ZHOU Shu-Jun

（The Key Laboratory of Horticultural Plant Growth，Development and Quality Improvement，the Ministry of Agriculture of the People’s Republic of China；Department of Horticulture，College of Agriculture and Biotechnology，Zhejiang University，Hangzhou 310058，China）

Online:2012-03-25 Published:2012-03-25

Abstract

Abstract: Hybridization between Asiatic lily‘Petit Brigitte’× Lilium tsingtauense was conducted with cut style pollination and embryo rescue. Fourteen progenies were obtained，and the parents and 4 progenies were analyzed using FISH with 45S rDNA as probe. The results showed that all of them are diploid（2n = 2x = 24）；‘Petit Brigitte’has 10 loci of 45S rDNA distributed on chromosome CH-1，CH-2，CH-5 and CH-7 and Lilium tsingtauense has 8 on CH-3，CH-4，CH-5 and CH-10 respectively，2n = 2x = 24 for Lilium tsingtauense and 2n = 2x = 24 for hybrids，respectively；all four progenies have 9 loci of 45S rDNA，unambiguously，coming from‘Petit Brigitte’and Lilium tsingtauense respectively.

Key words: Lilium, distant hybridization, rDNA, fluorescence in situ hybridization（FISH）

CLC Number:

S 682.2

REN Gui-Ling, ZHANG Xian-Xian, ZHOU Shu-Jun. Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH[J]. ACTA HORTICULTURAE SINICA, 2012, 39(3): 588-592.

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Hybridization and Identification Between Asiatic Lily and Lilium tsingtauense Using FISH

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