Abstract: A Nested-PCR method was developed to detect the bacterial pathogens of Anthurium andraeanum by using two sets of specific primers designed according to Xanthomonas campestris pv. dieffenbachiae gene sequences. All samples infected by Xanthomonas campestris pv. dieffenbachiae could be amplified by Nested-PCR，yielding one special band with size of 343 bp. The amplified sequences were presented in the Xanthomonas campestris pv. dieffenbachiae sequence X70380.1 in GenBank. Xanthomonas campestris pv. dieffenbachiae was detected by Nested-PCR from different parts of Anthurium andraeanum，and the distribution of pathogens in plants was uneven. Remarkably high amplification existed in old petioles，followed by stem-root joints，old leaves，young petioles，young leaves and spathe petiole，and the weakest amplification was in root，spathes and inflorescences.

Key words: Anthurium andraeanum, bacterial blight, Xanthomonas campestris pv. dieffenbachiae, Nested-PCR