Abstract: Tree peony‘Wulong Pengsheng’s lateral buds were used as explants. It was the first time to study the effect of cutting lateral buds on the adventitious buds differentiation. The influence of plant growth regulation on the adventitious buds induction，the multiplication and rooting was also studied. The results were as followed：（1）Disinfecting with 75% alcohol for 30 s，and then soaking with 0.1% corrosive sublimate for 7 min had good effect of disinfectation；（2）Compared with the control，the adventitious buds induction rate significantly increased through cutting，and the number of adventitious buds increased by 2.6–4.8 times，with the maximum reaching 96%；（3）The best medium for proliferation was WPM + 6-BA mg · L-1 + IAA 0.2 mg · L-1，with the proliferation of was 787.05%；（4）The rate of plantlets rooting was up to 81.33% on 1/2 WPM medium supplemented with NAA 1.0 mg · L-1 and IBA 4.0 mg · L-1. The plantlets were successfully transplanted to potting and the survival rate of transplanted plantlets was 90%.

Key words: tree peony, cutting, adventitious bud, proliferation, rooting