Abstract: The development process of spores of Adiantum flabellulatum in sterile culture condition and effects of different factors on spore germination and gametophyte development of A. flabellulatum were studied. The results showed that the spore germination was of the Vittaria-type and the gametophyte
development was of the Adiantum-type. Spore could not geminate in darkness. Proper time of deepfreeze was good for spore germination rate. After 20 days of spore sowing, the maximum germination rate was obtained in 1/4MS medium, which reached 22.7%; additional 15–45 g · L^-1 sucrose in culture medium were benefit spore germination，and sucrose was beneficial to gametophyte sexual differentiation；the sporophyte formation rate could reached 48.3% in MS + 30 g · L^-1 medium；additional auxins were not beneficial to sporophyte formation，but additional NAA 0.5 mg · L^-1 could promote gametophyte multiplication；sporophyte accommodation in sterile sand bottles could support a high alive rate about 90%.

Key words: Adiantum flabellulatum, spore, germination