Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (2): 213-220.

• 蔬菜 • Previous Articles Next Articles

Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale

REN Yan-rui;ZHANG Cheng-he*;SHEN Er-qiao;XUAN Shu-xin; SHEN Shu-xing

(College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001，China)

Received:2009-12-22 Revised:2010-01-25 Online:2010-02-25 Published:2010-02-25
Contact: ZHANG Cheng-he

Abstract

Abstract: In order to generate monosomic alien addition line of C-genome added the chromosome derived from A-genome, the triploid interspecies hybrid (CCA) and its parent Chinese kale (Brassica alboglabra Bailey) and Flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee) were used as the basic materials, by means of artificial hybridization, morphological and cytological examination and karyotype analysis, the seed setting of CCA × CC and CC × CCA, the chromosome number of their hybrids and the monosomic alien addition line of CC genome added a A-chromosome were identified. The main results showed as follows：① The seed setting of CCA × CC and CC × CCA was 0.28% and 0.63% respectively；② All of 88 progeny plants identified in CC × CCA were euploid（2n = CC = 18）；③
Aneuploids with different number extra chromosomes were existed in the identified 156 progeny plants of CCA × CC, in which one 2n + 1 plant and sixteen 2n + 2 plants were obtained；④ The monosomic alien addition line CC + A9 was preliminary determined by morphological marker identification and karyotype analysis for the 2n + 1 plant; ⑤ Meiotic observation for the addition line CC + A9 showed A9 chromosome having partial homology with a pair of C-chromosomes; ⑥ The alien chromosome A9 of the monosomic
addition line CC + A9 could be transmitted by male and female gametes with a percentage of 9.82% and 18.75% , respectively.

Key words: flowering Chinese cabbage, Brassica campestris L. var. utilis Tsen et Lee, Chinese kale, Brassica alboglabra Bailey, allotriploid, alien addition line

REN Yan-rui;ZHANG Cheng-he;SHEN Er-qiao;XUAN Shu-xin;SHEN Shu-xing. Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale[J]. ACTA HORTICULTURAE SINICA, 2010, 37(2): 213-220.

[1]	LI Zhijun, ZENG Jing, TAN Mingxi, XIE Jing. Embryogenesis Pathway of Chinese Kale Isolated Microspore and the Effect of 2,4-D on Embryo Formation [J]. Acta Horticulturae Sinica, 2021, 48(8): 1605-1618.
[2]	HU Wei, LIU Yuxi, ZHAO Qinzheng, CHEN Jinfeng, LOU Qunfeng. Creation and Identification of Cucumis sativus-C. hystrix Alien Addition Lines [J]. Acta Horticulturae Sinica, 2021, 48(7): 1349-1358.
[3]	ZHENG Hao, ZHANG Fen, JIAN Yue, HUANG Wenli, LIANG Sha, JIANG Min, YUAN Qiao, WANG Qiaomei, SUN Bo. Cloning and Function Identification of Dihydroflavonol 4-Reductase Gene BoaDFR in Chinese Kale [J]. Acta Horticulturae Sinica, 2021, 48(1): 73-82.
[4]	JIANG Ding, LI Xiangyang, LIU Zhenxiang, ZHENG Yansong, ZHANG Hua, and LI Guangguang. A New Chinese Kale Cultivar‘Jinsheng Jielan’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2965-2966.
[5]	ZHU Yunna1,2，ZHOU Xiaoxia1，LIANG Wenwen1，LIU Jianguo2，CHEN Riyuan1,，and SONG Shiwei1,. Cloning and Functional Analysis of BcAMT1;4 Gene Promoter in Flowering Chinese Cabbage [J]. ACTA HORTICULTURAE SINICA, 2020, 47(4): 675-685.
[6]	LI Guihua1，ZHANG Tianyu2，TANG Xiaobing3，CHEN Hancai1,*，LI Tingyao1，and ZHANG Yan1 . A New Chinese Kale Cultivar‘Zitai Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2777-2778.
[7]	LI Guihua1，CHEN Hancai1,*，ZHANG Yan1，LI Tingyao1，and CHEN Muxi2. A New Chinese Kale Cultivar‘Dongqiang Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2779-2780.
[8]	LI Tingyao，LI Guihua，ZHANG Yan，SHEN Zhuo，YANG Yi，and CHEN Hancai*. A New Chinese Kale Cultivar‘Qiubao Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2781-2782.
[9]	SONG Kanghua，JIA Zhiwei，CHANG Jinmei，SUN Manli，and ZHANG Lubin*. Lignification Induced by Ethephon and Related Gene Expression in Postharvest Flowering Chinese Cabbage at Low Temperature [J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 775-783.
[10]	XIA Yanshi1，ZHANG Runlin1，LU Yupeng1，LI Ronghua1，LI Guangguang2，ZHANG Hua2，and GUO Peiguo1,*. Association Analysis of Quality Traits with SSR Markers in Flowering Chinese Cabbage [J]. ACTA HORTICULTURAE SINICA, 2019, 46(10): 1960-1972.
[11]	ZHENG Aihong，ZHANG Fen，JIANG Min，YUAN Qiao，JIANG Leiyu，CHEN Qing，TANG Haoru，and SUN Bo**. Targeted Editing of BoaZDS by CRISPR/Ca9 Technology in Chinese Kale [J]. ACTA HORTICULTURAE SINICA, 2019, 46(1): 57-64.
[12]	HUANG Hongdi*，ZHANG Hua，CAO Jiansong，LI Guangguang，and ZHENG Yansong. A New Flowering Chinese Cabbage Cultivar‘Yutian 2 Caixin’ [J]. ACTA HORTICULTURAE SINICA, 2017, 44(s2): 2677-2678.
[13]	XIAO Xufeng1，ZHANG Yi1，WU Zhiming2，and YANG Yingui1,*. Cloning，Sequence Analysis and Expression Profile of Protein Arginine N-methyltransferase Gene BrcuPRMT5 in Flowering Chinese Cabbage [J]. ACTA HORTICULTURAE SINICA, 2017, 44(6): 1181-1188.
[14]	CHEN Han-cai1，LI Gui-hua1,*，ZHANG Yan1，LIU Kai2，and LI Ting-yao1. A New Chinese Kale Cultivar‘Donglü Jielan’ [J]. ACTA HORTICULTURAE SINICA, 2016, 43(S2): 2741-2742.
[15]	LI Rong-hua1，WANG Zhi-liang1，CHEN Jing-fang1，XIA Yan-shi1，GUO Pei-guo1,*，ZHANG Hua2，and Kadambot Siddique3. Analysis of SSR Information in Transcriptome and Their Usability in Flowering Chinese Cabbage [J]. ACTA HORTICULTURAE SINICA, 2016, 43(9): 1816-1824.

Chromosome Number Identification and Generation of Monosomic AlienAddition Line in the Triploid Interspecies Hybrid of Flowering ChineseCabbage–Chinese Kale

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments