Abstract: Postharvested Flammulina velutipes were packed with nano-packaging material or polyethylene（PE）material，and then stored at（2 ± 1）℃. To explore the right atmosphere conditions of cold storage for F. velutipes，O2 and CO2 contents of different packages and active oxygen metabolism were detected，and the changes of the quality were observed. The results showed that nano package could maintain hypoxia and hypercapnia storage environment（CO2 11.5%，O2 1.6%），so as to keep the appearance of mushroom quality，prolong the storage time from 12 to 20 days. Meanwhile，the microenvironment of nano package effectively inhibited the levels of superoxide anion and hydrogen perotride（H2O2），and decreased malonaldehyde（MDA）content，while enhanced the activities of superoxide dismutase（SOD），catalase（CAT），peroxidase（POD）and ascorbate peroxidase（APX）. It suggested the condition of CO2 11.5% and O2 1.6% is better than CO2 9.7% and O2 2.4% for F. velutipes cold storage.

Key words: Flammulina velutipes, nano-packaging material, modified atmosphere storage