ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (9): 1291-1298.
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YANG Feng-juan1,2;WEI Min1,2;SU Xiu-rong3;WANG Xiu-feng1,2
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Abstract: The changes of calcium level in rootmeristem region of cucumber seedling (‘XintaiMici’) under different NO3- concentrations stress were determined by the imp roved cytochemical method of calcium pyroantimonate p recip itation. After 7 days of treatment, under op timum NO3- (14 mmol·L -1) conditions,calcium antimonite deposits, which indicates calcium distribution, were mainly localized in cellmembrane and vacuoles, as a storeroom of Ca2+, and smaller amounts of calcium p recip itates randomly resided in mitochon2dria, cytop lasm and nucleus. But under excessive NO3-stress, the distribution of Ca2+ in subcellular was changed greatly. After 7 days treated with 56 and 140 mmol·L-1 NO3-, Ca2+level in the intercellular spaces, cytop lasm and mitochondria elevated. With the increasing NO3-concentrations, the shape and amount of mitochondria and vacuoleswere changed greatly. Higher NO3-concentrations (182 mmol·L-1) caused a series of disorders of metabolic p rocesses and serious damages of membrane system of organelles in cucumber seedling roots, thus, Ca2+deposits in each compartments became smaller significantly. The result indicated that the change of Ca2+ from vacuoles to hyalop lasm can reduce the NO3-stress in cucumber seedlings.
Key words: cucumber seedling, NO3-stress, calcium pyroantimonate precipitation, cytochemistry, Ca2+
CLC Number:
S 633.1
Q 945.78
YANG Feng-juan;WEI Min;SU Xiu-rong;WANG Xiu-feng;. The Differences of Subcellular Ca2+ Distribution in Root Meristem Region ofCucumber Seedling Under Different NO3- Concentrations Stress[J]. ACTA HORTICULTURAE SINICA, 2009, 36(9): 1291-1298.
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https://www.ahs.ac.cn/EN/Y2009/V36/I9/1291