Abstract: The type and character of floral organ variation in Juglans regia L. were observed, and the anatomy of hermaphroditic development was also analyzed through paraffin section. The 2942 seedlings from cross combination were raised using 6 precocious forms, and 2 later mature forms.Among 2648 seedlings from artificial cross by precocious forms and open pollination of precocious forms, 20.34% of the seedlings were produced flowers in the third years .The 294 seedlings from later mature forms produced no flowers in the third years. 12 seedlings of the 538 precocious cross seedlings were observed on floral organ variations. The variation type includes Hermaphrodite flower, spike inflorescences, branch spike inflorescences, male and female flowers in one spike inflorescence, rosette male inflorescences. The study on floral organ variation was kept from 2003 to 2008. Stable inheritance of floral organ variation only appeared in C3 cross seedling from Xin 1 × Xiangling. The floral organ variation was found in adventitious shoots of Xiangling cultivar. The anatomy of Hermaphrodite flower development procedure was: inflorescence primordium - flower primordium - bract primordium - stamen primordium - stigma primordium - ovary primordium. The floral organ variation only took place in secondor shoots and secondor flowers. The walnut with floral organ variation is a valuable material to study the earlier fruiting character and the floral organ development of walnut.

Key words: Walnut, Juglans regia L., Precocious trait, Floral variation, Hermaphrodite flower