Development of Molecular Markers IRAP and REMAP in Eggplant

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (9): 1363-1367.

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Development of Molecular Markers IRAP and REMAP in Eggplant

WANG Li-ying^1,2,DU Yong-chen^1*,ZHANG Bin²,SHI Yao²,WANG Qiu-jin³,and ZHAO Fu-kuan³

(¹Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences, Beijing 100081 ,China；²TianJin Kernel Vegetable Research Institute , Tianjin 300384 , China ;³Department of Biological Technology , Beijing University of Agriculture , Beijing 102206 , China)

Received:2008-03-04 Revised:2008-08-11 Online:2008-09-25 Published:2008-09-25
Contact: DU Yong-chen

Abstract

Abstract: Primers were designed based on LTR reserve region of Bare-1 and Tto1 in tobacco and barley．14 varieties of eggplant were used as experimental materials for PCR amplification．Agarose and polyacrylamide gel electrophoresis were adopted to detect amplification products and the technique of IRAP and REMAP molecular marker was constructed．Polymorphic fingerprints were obtained using two methods (IRAP and REMAP)，new methods were developed for variety appraisal and fingerprint in eggplant．

Key words: eggplant, retrotransposon, IRAP, REMAP

CLC Number:

S 641.1

WANG Li-ying;DU Yong-chen;ZHANG Bin;SHI Yao;WANG Qiu-jin;and ZHAO Fu-kuan. Development of Molecular Markers IRAP and REMAP in Eggplant[J]. ACTA HORTICULTURAE SINICA, 2008, 35(9): 1363-1367.

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Development of Molecular Markers IRAP and REMAP in Eggplant

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