Abstract:

MADS-box genes play important role in various plant development processes，especially in the development of floral organs．The flowering-related MADS-box gene, named as NTMADS1 (GenBank accession number：EF421828), was cloned from the first strand of N. tazetta var. chinensis cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 879 bp long and contained an open reading frame of 693 bp coding a polypeptide of 230 amino acids which had a typical MADS-box domain. Homology analysis showed that the deduced NTMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with Asparagus virgatus to 89%. Phylogenetic tree analysis also indicated that NTMADS1 belongs to AG subfamily.

Key words: Narcissus tazetta var. chinensis, MADS-box gene, Phylogenetic tree analysis