A Modified Method of Amplifying the 5′-end cDNA Sequence

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (10): 1533-1538.

• 新技术与方法 • Previous Articles Next Articles

A Modified Method of Amplifying the 5′-end cDNA Sequence

XIA Rui^1,3;LU Wang-jin2;and LI Jian-guo^1*

(¹China Litchi Research Center, South China Agricultural University, Guangzhou 510642, China; ²Guangdong Provincial Key Lab of Post-harvest Science of Fruit and Vegetables, Guangzhou 510642, China; ³Institute of Fruit Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China)

Received:2008-05-29 Revised:2008-09-12 Online:2008-10-25 Published:2008-10-25
Contact: LI Jian-guo

Abstract

Abstract:

In order to obtain the full-length sequence of genes more efficiently, a modified method of amplifying the 5′ cDNA sequence, named as modified TDT tailing amplified method, was designed in this experiment. Many strategies are used in this method including TdT tailing, anchored PCR, nested PCR, touchdown PCR. Compared with a regular method using a kit (5′-Full RACE Core Set, TaKaRa), the modified method has many advantages, such as simple principle, easy operability, high amplified specificity, and reliable results. Moreover, it costs less money and time. Therefore, it is worth popularizing in ordinary laboratories.

Key words: TdT, RACE, touchdown PCR, Dl-Exp1

CLC Number:

Q 943.2

XIA Rui;LU Wang-jin;and LI Jian-guo. A Modified Method of Amplifying the 5′-end cDNA Sequence [J]. ACTA HORTICULTURAE SINICA, 2008, 35(10): 1533-1538.

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A Modified Method of Amplifying the 5′-end cDNA Sequence

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