Abstract: The development process and spore sterile culture of Pteris wallichiana Agardh. were studied respectively. The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type. The maximum germination rate was obtained in 1/2MS medium, which reached 83.3%; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached 55.4%; the transplant survival rate of sporophyte in vitro reached above 80%. The research indicates that the utilization of the spore sterile culture of P. wallichiana for its large-scale production is possible.

Key words: Pteris wallichiana Agardh., spore, sterile culture