Floral Organ in Vitro Culture of Clivia miniata Regei

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (6): 1563-1568.

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Floral Organ in Vitro Culture of Clivia miniata Regei

XING Gui-mei, BI Xiao-ying, and LEI Jia-jun^*

(College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China)

Received:2007-04-19 Revised:2007-09-01 Online:2007-12-25 Published:2007-12-25

Abstract

Abstract: The petal, filament and ovule of Clivia miniata Regei 'Youjiang' were used as explants for in vitro culture. The results showed that the explants disinfected with 0.1% HgCl₂ for 10 min were most effective with the average contamination rate of 9.9%.The rates of induction and differentiation of three kinds of floral organ explants were different. The petals had the highest induction and differentiation rates of 15.6% and 57.1%, respectively. There was direct relationship between percentage of induction and differentiation of petals and size of flower buds, concentration of hormone and sucrose. The rates of callus induction and differentiation were the highest when the length of flower bud was 0.6-1.0 cm. The optimum medium was MS+2,4-D 2.0 mg·L^-1+BA 1.0 mg·L^-1+NAA 0.5 mg·L^-1+3.0% sucrose. The rate of induction and differentiation of floral organ explants was much higher while the concentration of sucrose was 3%.

Key words: Clivia miniata Regei, Floral organ, Tissue culture

CLC Number:

S 682.1⁺3

XING Gui-mei;BI Xiao-ying;and LEI Jia-jun. Floral Organ in Vitro Culture of Clivia miniata Regei[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1563-1568.

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Floral Organ in Vitro Culture of Clivia miniata Regei

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