Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 865-870.

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Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach

WANG Cheng¹;CAO Hou-nan^1,3;ZONG Cheng-wen¹;ZHAO Cheng-ri²;ZHUANG De-feng¹;P IAO Ri-zi¹;ZHAO Kai¹

(¹Agricultural College, Yanbian University, Longjing, Jilin 133400, China; ² Faculty of Applied Biological Sciences, Gifu University, Gifu 5011193, Japan)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: In this study, a RAPD marker (OPW03-900) linked to pollen fertility gene was screened
from 180 RAPD arbitrary p rimers in 52 F₁ individuals from the cross‘Chongyanghong’ ×‘Okuba’, pollen
fertility/ sterility poolwas constructed respectively by using RAPD technique and BSA method. After repeating
tests and checking up individuals of population, thismarker could only be amp lified in the pollen fertility indi
viduals ( excep t recombinants) , the linkage distance was 5.80 cM with the pollen fertility/ sterility locus. This
band was collected and sequenced to synthesize a pair of SCAR p rimerwhich was used to amp lify the individu
als of the F1 p rogeny, the locus of the special band and the number of recombinantswere the same as the result
of RAPD amp lification, the size of thismarkerwas 906 bp, which was designated as SCW03-906, indicating
the RAPD marker has been successfully converted into a SCAR markerwhich linked to pollen fertility gene in
peach. This sequence was adop ted by GenBank, the logging number wasDQ659676.

Key words: RAPD marker, BSA, SCAR marker, Pollen fertility gene

WANG Cheng;CAO Hou-nan;ZONG Cheng-wen;ZHAO Cheng-ri;ZHUANG De-feng;P IAO Ri-zi;ZHAO Kai. Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 865-870.

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Identif ication of RAPD Marker Linked to Pollen Fertility Gene and Conversion to SCARMarker in Peach

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