Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 637-643.

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Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)

DAI Su-ming^1,2 ; ZHOU Cheng-ai^1,2 ;XIE Bing-yan^1*
, XIAO Qi-ming² ;YANG Yu-hong¹ ;YIN Li-rong^1,2

( ¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²College of Plant Protection, Hunan Agricultural University, Changsha 410128, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: The complete CYP92A was amp lified by RT2PCR and RACE, which expressed in pepper (Cap
sicum annuum L. ) leaf was rapidly and transiently induced by β-aminobutyric acid (BABA). The analysis of
bioinformatics showed that CYP92A gene encoded a 5092 amino acid protein which contained a conserve heme2
binding region of cytochrome P450. Therefore, it was confirmed that CYP92A is cytochrome P450. By phyloge
netic analysis, it was belived to belong to 92A sub-family of P450. According to researches of other 92A sub
family genes reported, it is speculated conferred that CYP92A may play a role in plant defence response. Based
on bioinformatics analysis of protein struction, the p roperty of CYP92A in catalytic reaction was also discussed in
this paper. This study had significance for research on indued2resistance mechanisms of BABA.

Key words: Capsicum annuum L., Cytochrome, BABA, B ioinformatics, Plant defence response

DAI Su-ming;;ZHOU Cheng-ai;;XIE Bing-yan;XIAO Qi-ming;YANG Yu-hong;YIN Li-rong;. Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 637-643.

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Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)

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