Abstract: Four combinations of Canadice ×Venus Seedless, Remaily Seedless ×Venus Seedless, Selfing of Venus Seedless and selfing of Lakemont Seedless were used as test materials in this experiment. This
experimentmainly studied the influence of inoculating time and different kinds ofmedia on the effect of embryo
rescue of seedless grape by the orthogonal experiment design of L₂₅ (5⁵ ). Results showed that the most important factor influencing the embryo rescue of seedless grape was the inoculating time, and the influence level of
other factorswas different among combinations. The op timum inoculating time of the embryo was 45 - 60 days after blooming and the seedling rate reached 13.64% - 75.23%. It also showed that certain treatments of ovule increased the germination rate of ovule, among them the treatment of crosscut and low temperature increased the germination rate of ovule significantly.

Key words: Seedless grape, Embryo rescue, Influence factors