In Vitro Ovary Culture of Cardiocrinum giganteum

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (1): 197-200.

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In Vitro Ovary Culture of Cardiocrinum giganteum

LI Shou-li^1,2 ; SHI Lei^1*
; ZHANG J in-zheng¹ ; ZHUANG Ping³

(¹ Institute of B otany, the Chinese Academy of Sciences, Beijng 100093, China; ² Graduate School of the Chinese Academy of Sciences, Beijing 100039, China; ³ West China Subalpine Botanical Garden, Institute of Botany, the Chinese Academy of Sciences,Dujiangyan, Sichuan 611830, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract: An experiment was carried out on the induction of bulbulets and the plant regeneration by
using the ovaries of Cardiocrinum giganteum as exp lants. The results indicated that BA and KT were the main
factors in ovary explant differentiation. The explants differentiated into calluses when the concentration of BA
and KT was between 0.1 and 1.0 mg/L and budswere induced when the concentration was bewteen 2.0 and
4.0 mg/L, and when higher than 4.0 mg/L, leaves formed. The best basic media for the dedifferentiation
were N6 and B5. The bestmedium for bulbulet induction from calluswasMS + 0.1 - 0.5 mg/L NAA + 2.5
mg/L BA + KT 2.5 mg/L + 10% sucrose + 0.7% agar. The ratio of root induction from calluswas 100% on
the medium of 1/2MS + 3% sucrose + 0.7% agar + 1% active carbon. After acclimatization for one week,
the bulblets were transp lanted into greenhouse and all grew well.

Key words: Cardiocrinum giganteum, Tissue culture, Ovary

LI Shou-li;;SHI Lei;ZHANG J in-zheng;ZHUANG Ping. In Vitro Ovary Culture of Cardiocrinum giganteum[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 197-200.

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In Vitro Ovary Culture of Cardiocrinum giganteum

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