Abstract: Several factors affecting isolated microspore culture of non-heading Chinese cabbage
(Brassica campestris ssp. chinensis) were studied in experiments. The results from mono2factor experiments
showed that there were significant differences in the frequency ofmicrospore-induced embryos among different
genotypes. In the low temperature treatment, no significant differences were found during 0 - 5 days, while
the frequencies were obviously lower when the period was longer than five days. There were no significant
differences in high temperature when the incubation time was within 12 - 60 h, but the frequencies of
microspore-induced embryos were much lower over this duration. The addition of auxins (NAA ) and the
cytokinins ( 6-BA) in NLN medium showed no significant effect on the frequencies of microspore-induced
embryos, but when the concentration was excessive, the frequencies decreased. The presence and
concentration of activated charcoal had a great influence on the frequency ofmicrospore-induced embryos. Mo
reover, four-factor analysis of genotype, auxin, cytokinins and activated charcoal concentrations indicated that
there were significant differences among different genotypes and activated charcoal concentrations,
respectively, and also significant differences among these two interactions, while other interactions remained
non-significant.

Key words: Brassica campestris ssp. chinensis, Microspore culture