Abstract: The leaf, floral stem, recep tacle, ovary, scale, axillary bud of 4 tulip cultivars, ‘Kees Nelis’, ‘Merry Widow’, ‘Parade’, and‘Golden Parade’, were used as explants in vitro culture on the media of MS + 2, 4-D 0.1 mg·L ^{- 1} , MS + 2, 4-D 0.5 mg·L ^{- 1} , MS +BA 1.0 mg·L ^{- 1} , MS +BA 5.0 mg·L ^{- 1} ,
MS +BA 2.0 mg·L ^{- 1} + IAA 0.5 mg·L ^{- 1} in the experiment. The results showed that the percent of bulblet induction was highest when receptacle was used as explant, with 56.7% on MS + 2, 4-D 0.5 mg·L ^{- 1} , followed by floral stem with 33.3% on the same medium. The average number of the regenerating bulblets was 10.7 per receptacle explant and 19.9 per floral stem segment explant. The scale segment and axillary bud were difficult and with a longer time to regenerate bulblet. The leaf segment and ovary failed to regenerate bulblet in the experiment.

Key words: Tulip, Tulipa gesneriana L., Tissue culture, Callus, Regenerating bulblet