Abstract: Through crossing between seedless grapes and Chinese wild grapes we got four group hybrid grape seedlings. The best time to harvest the hybrid grapes ovule for embryo rescued were as follows: 45 d after pollination are (1) Flame seedless with Vitis amurensis, (2) Ruby seedless with Olmo seedless, (3)Ruby seedless with Beichun, (4) Olmo seedless with Vitis amurensis; 30 d after pollination is (5) Changsui saltanina seedless with Vitis amarensis; 35 d after pollination is (6) Self-pollinated saltanina. Nistschs medium with additional 0.5 mg·L ^{- 1} 6-BA + 0.5 mg·L^-1 GA₃ + 2.5 mg·L^-1 IBA + 0.1 mg·L^-1 ZT was suitable for cross (1) , (3) and (5) , but Nistschs medium with additional 0.5 mg·L^-1 6-BA + 0.5 mg·L^-1GA₃ + 2.0 mg·L^-1 IBA + 0.1 mg·L^-1 ZT was suitable for cross (2) , (4) and (6). For embryo regeneration Nistschs medium with additional 2.0 mg·L^-1 IBA + 1.0 mg·L^-1 6-BA + GA₃ 0.2 mg·L^-1 was suitable for cross (1) , (2) , (3) , (5) and (6) , but for cross (4) medium with 1.5 mg·L^-1 IBA + 0.2 mg·L ^{- 1} GA₃ + 1.0 mg·L ^{- 1} 6-BA was more suitable. 1/2MS medium adding 0.15 mg·L ^{- 1} IBA + 0.02 mg·L ^{- 1} 6-BA were suitable for cross (1) and (5) to acquire embryo rescue plants, but 1 /2MS medium with addition 0.1 mg·L ^{- 1} IBA + 0.02 mg·L ^{- 1} 6-BA was suitable for cross (2) and (4).

Key words: Seedless grape, Chinese wild grape, Hybrid, Embryo rescue