Cloning and Sequence Analysis of Citrus Calmodulin cDNA

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 1075-1078.

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Cloning and Sequence Analysis of Citrus Calmodulin cDNA

Yue Hailin^{1, 2};Meng Haijun¹;Deng Xiuxin¹;Peng Shupang^2*

(¹National Key Laboratory of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan, Hubei 430070, China;²College of Forestry and Horticulture, Huazhong Agriculture University, Wuhan, Hubei 430070, China)

Received:2005-12-20 Revised:2006-03-02 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: Using the first strand cDNA from the immature fruits of Citrus unshiu Marc. as template, we amplified and cloned the cDNA of Citrus calmodulin gene in this study. The PCR primers were designed according to the sequences of barley calmodulin gene obtained from GenBank (Accession No. M27303). The PCR product was cloned into PMD182T vector and then sequenced. Sequence analysis indicated that the cDNA of Citrus calmodulin gene contains 453 nucleotides coding for 148 amino acid residues. The nucleotide sequences and their deduced amino acid sequences of the Citrus calmodulin cDNA show an identity of above 83% and 97% , respectively, to those of barley and soybean. Southern dot blot analysis using the cloned cDNA as probe confirmed the results of sequencing analysis.

Key words: Citrus, Calmodulin, cDNA cloning, Sequence analysis

Yue Hailin;;Meng Haijun;Deng Xiuxin;Peng Shupang. Cloning and Sequence Analysis of Citrus Calmodulin cDNA[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1075-1078.

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Cloning and Sequence Analysis of Citrus Calmodulin cDNA

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