Abstract: Effects of pre-fertilization barriers including the viability, germination ability, preservation conditions of pollen and pollination methods on interspecific Lilium crosses was studied in L. pumilum, ‘Siberia’, ‘White Fox’, ‘Pink Perfection’, ‘Ceb Dazzle’and‘Victoria Falls’. The results showed that the
op timum concentration of sucrose contributes to the pollen germination. Two techniques of pollination were studied comparatively for pollen germination. It appeared that pollen tubes stopped growth and the pollen was not able to germinate on cut-style 48 h after pollination. The use of in vitro pollination showed that the pollen tubes can penetrate ovary into ovules 24 hours after pollination and the strong swelling of ovary happened 45 days after pollination. The hybrid seedlings were attained by embryo rescue.

Key words: Lilium, Pre-fertilization barrier, Pollen viability, Germination, Cut-style