Studies of Somatic Embryo via Cotyledon Induction in Cucumis melon L.'Hetao’

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (6): 1108-1110.

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Studies of Somatic Embryo via Cotyledon Induction in Cucumis melon L.'Hetao’

Han Hongyan;Li Lianguo;Jia Yuchen;Zhang Qingmei

(Agricultural College, Inner Mongolia Agricultural University, Huhhot 010019, China)

Received:2005-01-11 Revised:2005-05-10 Online:2005-12-25 Published:2005-12-25

Abstract

Abstract: The main factors of somatic embryo on Cucumis melon L.‘Hetao’from cotyledon explants were studied and embryogenesis was observed. The results indicate that the highest regeneration was occurred on MS medium containing 60 g/L sugar and supp lemented with 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA. Daily cotyledon explants were cultured one week in darkness, followed by another week photoperiod before transfering to embryos development medium, produced more mature embryos. Somatic embryos were formed from the inside or the surface of callus, the surface of explant, adventitious root and meristem of explant vascular.

Key words: Cucumis melon L., Somatic embryo, Regeneration plant

CLC Number:

S 652

Han Hongyan;Li Lianguo;Jia Yuchen;Zhang Qingmei. Studies of Somatic Embryo via Cotyledon Induction in Cucumis melon L.'Hetao’[J]. ACTA HORTICULTURAE SINICA, 2005, 32(6): 1108-1110.

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Studies of Somatic Embryo via Cotyledon Induction in Cucumis melon L.'Hetao’

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