Abstract: The zygotic embryos, obtained from crossing between Chinese persimmon ‘Mopanshi’ (Diospyros kaki Thunb. ‘Mopanshi’) and Japanese cultivar‘Nishimura Wase’, were rescued under in vitro conditions. The effect of nitrogen (N) concentration in MS medium, and the effects of sucrose, 6-BA and
IBA concentrations on the rates of sprouting and lateral rooting and plantlet formation were investigated using Orthogonal design at 3 levels. The results showed that sucrose concentration was the most effective factor, then, followed by IBA and 6-BA in these four factors. The concentrations of N in MS medium exhibited no considerable effect to rates of sprouting and lateral rooting and plantlet formation, but showing prominent effect to leaf color. This study also demonstrated that the optimum medium for embryo rescue was MS basal medium containing 3 /4N + 6-BA 0.1 mg·L ^{- 1} + IBA 0.02 mg·L ^{- 1} + sucrose 50 g·L ^{- 1}.

Key words: Persimmon, Zygotic embryo, Rescue culture