Application of SSR Markers in Hybrid Seed Purity Test of Melon

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (5): 902-904.

• 研究报告 • Previous Articles Next Articles

Application of SSR Markers in Hybrid Seed Purity Test of Melon

Ai Chengxiang1;Lu Lu1, 2;Ma Guobin3;Liu Zhixin1

( ¹ Institute of Bioscience and Biotechnology, State Key Lab for Tropical Crops Biotechnology, Chinese Academy of Tropical Agriculture Science, Haikou 571101, China; ²College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; ³Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China)

Received:2004-11-25 Revised:2005-05-25 Online:2005-10-25 Published:2005-10-25

Abstract

Abstract: The polymorphism of 2 melon hybrids ( ‘Dongfangmi 1’and ‘01231’) and its parental lines were analyzed with SSR marker system. Eight out of 23 pairs of SSR p rimerswere selected, which gave stable and polymorphic amplification profiles in hybrids and their parents. The bands of hybrid melon were
complement types of their parents, which were adap ted to examine purity of hybrid melon seed. Primer M6 was selected for SSR identification with 100 single seeds of‘Dongfangmi 1’, and p rimerM17 for‘01231’. The purity was 100% and 96% , respectively, which were app roximate to their field purity (99.6% and 96.2% , respectively). It suggested that SSR technique haswide prospective in hybrid seed purity test of melon.

Key words: Melon, SSR marker, Polymorphic amplification, Purity test

CLC Number:

S 652

Ai Chengxiang;Lu Lu;;Ma Guobin;Liu Zhixin. Application of SSR Markers in Hybrid Seed Purity Test of Melon[J]. ACTA HORTICULTURAE SINICA, 2005, 32(5): 902-904.

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Application of SSR Markers in Hybrid Seed Purity Test of Melon

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