Abstract: Using the embryo, stem-segment of new shoots as the exp lants, in vitro culture and high frequency propagation of sweet osmanthus were studied. The results showed that the optimizing media for various stages were as follows: (1) Initial medium for embryo: MS +BA 1.00 mg·L ^{- 1} + 3% sucrose; (2) Initial
medium of stem-segment for new shoots: LMc + KT 8.00 mg·L ^{- 1} +NAA 0.10 mg·L ^{- 1} + 3% sucrose; (3) Clump shoot regeneration medium: LMc + TDZ 0.50 mg·L ^{- 1} +NAA 0.10 mg·L ^{- 1} + 3% sucrose; ( 4) Rooting medium: 1 /2MS +NAA 2.00 mg·L ^{- 1} + 3% sucrose. Using humus soil as culture substrate, the
survival percentage of plantlets could reach 80%.

Key words: Sweet osmanthus, In vitro culture, Rapid propagation