Abstract: Effects of several factors such as medium, cutting materials and plant growth regulators on invitro culture and rapid propagation of Callistem onrigidus R. Br were investigated. Using the leaf and axillary bud as explant, a method for high frequency propagation of callistem onrigidus R. Br was established. Results showed: ( 1) MS medium supplemented with 6-BA 1.5 mg·L ^-1 and NAA 0.5 mg·L ^-1 was aptimized for callus induction of leaf. The crowd of bud on callus was differented with the ratio of 69.2%. On MS medium supplemented with 6-BA 1 - 1.5 mg·L ^{- 1} and NAA 0.5 mg·L ^{- 1} , the crowd of bud was started with the ratio of 77%. ( 2) The explants diffedrentiated a large number of young buds on MS medium containing 6-BA 1 mg·L ^{- 1} and NAA 0.25 mg·L ^{- 1}. On this medium shoot multiplication occurred at a 16.7 - fold rate. (3) The shoots were rooted on the 1/2 strength MS medium supplemented with NAA 0.25 mg·L ^{- 1} and the rate of rooting was 96.1%. The shoots could be directly rooted in transplantation medium with up to 90% survival.

Key words: Callistem onrigidus R. Br, Plant tissue culture, In vitro propagation, Callus