RAPD Analysis of Germplasm Resources on Canna

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (02): 273-277.

• 研究论文 • Previous Articles Next Articles

RAPD Analysis of Germplasm Resources on Canna

Huang Guotao¹;Ouyang Dimei²;Xiang Qibai¹;Zhu Weihua²;Zhang Shouzhou³

(¹College of Landscape, Nanjing Forestry University, Nanjing 210037, China; ² Shenzhen Urban Garden Administration, Shenzhen 518035, China; ³ Shenzhen Fairy Lake Botanical Garden, Shenzhen 518004, China)

Received:2004-07-01 Revised:2004-10-22 Online:2005-04-25 Published:2005-04-25

Abstract

Abstract: Taxonomy of four Canna species and 52 cultivarswere analyzed using random amplified polymorphic DNA. 27 p rimers were screened from 125 arbitrary 102 mer primers, and a total of 223 DNA bands were amplified, 140 of which (62.78% ) were polymorphic. The average numbers of DNA bands amplified
by each primer were 8.26. There were specific amplified bands in three species and seven cultivars, and these bands can be used in Canna germplasm identification. The dendrogram constructed by UPGMA showed that the 56 individualswere classified into four groups. Four specieswere grouped in dependently and 52 cultivars were classified into Canna ×generalis Bailey and C. ×orchioides Bailey. This conclusion consisted with the traditional Canna taxonomy. Phylogenetic relationship of Canna germplasm was also discussed.

Key words: Canna Linn., RAPD, Taxonomy, Phylogenetic relationship

CLC Number:

S682.2⁺2

Huang Guotao;Ouyang Dimei;Xiang Qibai;Zhu Weihua;Zhang Shouzhou. RAPD Analysis of Germplasm Resources on Canna[J]. ACTA HORTICULTURAE SINICA, 2005, 32(02): 273-277.

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RAPD Analysis of Germplasm Resources on Canna

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