Abstract: The study aimed on the selection of embryonic callus of Kalanchoe blossbeldiana，the induction of embryoid，morphogenesis of embryoid and regeneration．The grain-like，light yellow and loose callus was embryonic callus. During the inducing of embryoid，the culture mediums with different growth regulator concentration were used．Finally the culture medium with the highest of reduction frequency was MS+BA 2 mg·L^-1+NAA 0.2 mg·L^-1+Sucrose 30 g·L^-1 +Active carbon 4 g·L^-1 (185 embryoid per gram)．The regeneration medium Was MS+Sucrose 30 g·L^-1．The appearance and growing of embryoid were also observed through parafin slices of callus．The development procedure of embryoid Was procel of embryoid，globular stage，heart-shape stage and cotyledonary stage

Key words: Kalanchoe blossfeldiana, Embryonic callus, Embryoid, Regeneration