Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid ofEggplant

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (2): 233-235.

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Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid ofEggplant

Lian Yong ;Liu Fuzhong;Chen Yuhui;Song Yan;Zhang Songlin ; Darasinh Sihachakr

( Institute o fVegetabales and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China； Morphogenese Vegetable Experimentale，Universite Paris Sud，BAT．360，91405 ORSAY CEDEX，France)

Received:2003-09-12 Revised:2003-12-24 Online:2004-04-25 Published:2004-04-25

Abstract

Abstract: Somatic fusion hybrid DSa(S．torvum Sw×S．melongena L．‘Dourga’)microspore were cultured by using isolated microspore culture． The result show that microspore dediferentiation ability was markedly improved by using the treatment of anther under the high temperature conditions of 36 ℃ for
continuous 6 days． The best development period for microspore culture is monokaryotie stage；The suitable medium for microspore culture is KM with PEG 250 mg/L，2，4-D 0.2 mg/L，ZT 0.5 mg/L，NAA 1 mg/L and glucose 6.5%；The suitable medium for regenerated plantlet is MS with ZT 2 mg/L，IAA 0.1 mg/L，sugar 2% and agar 7 g/L．

Key words: Eggplant, Somatic hybrid, Isolated microspore culture, Plantlet regeneration

CLC Number:

S641．1

Lian Yong;Liu Fuzhong;Chen Yuhui;Song Yan;Zhang Songlin;Darasinh Sihachakr. Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid ofEggplant[J]. ACTA HORTICULTURAE SINICA, 2004, 31(2): 233-235.

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Plantlet Regeneration by Isolated Microspore Culture of Somatic Hybrid ofEggplant

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