Using Protoplast Electrofusion Technology to Obtain Interspecific SomaticFusion Hybrid of Eggplant

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (1): 39-42.

• 研究论文 • Previous Articles Next Articles

Using Protoplast Electrofusion Technology to Obtain Interspecific SomaticFusion Hybrid of Eggplant

Lian Yong^1,2; Liu Fuzhong¹; Feng Dongxin¹;Song Yan¹;Chen Yuhui¹;Zhang Songlin¹;Darasinh Sihachakr 　

(¹ Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Morphogenese Vegetale Experimentale, Universite Paris Sud, Bat. 360, 91405 orsay cedex, France)

Received:2002-12-24 Revised:2003-07-25 Online:2004-02-25 Published:2004-02-25

Abstract

Abstract: We obtained regenerated tetroaploid plants from the protoplast electrofusion between Solanum melongena (Dourga) and S.torvum or S.aethiopicum. The ploid level was confirmed by chromosome detection. Fluorescent immunoassay proved that the plants were regenerated from interspecific somatic fusion. Inoculation with Pseudomonas solanacearum indicated that the somatic hybrids carried the resistance gene from the wild relatives. The somatic hybrids were more like the wild relatives in the open field.

Key words: Eggplant, Protoplast, Fusion, Somatic hybrid

CLC Number:

S 641.1

Lian Yong;Liu Fuzhong;Feng Dongxin;Song Yan;Chen Yuhui;Zhang Songlin;Darasinh Sihachakr 　. Using Protoplast Electrofusion Technology to Obtain Interspecific SomaticFusion Hybrid of Eggplant[J]. ACTA HORTICULTURAE SINICA, 2004, 31(1): 39-42.

[1]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, GONG Chao. A New Eggplant Cultivar‘Xinfeng 3’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 227-228.
[2]	CAI Peng, FANG Chao, LI Yuejian, LIU Duchen, LIU Xiaojun, LIANG Genyun. A New Eggplant Cultivar‘Tianjiao’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 229-230.
[3]	TAN Jie, HUANG Shuping, CHEN Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 99-100.
[4]	HUANG Shuping, TAN Jie, Chen Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 6’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 101-102.
[5]	YANG Yang, TIAN Shibing, WANG Yongqing, JIANG Changchun, ZOU Min, TAO Tao, ZHOU Shanshan, WANG Zhijin, BAO Zhongxian, and TANG Xiaohua. A New Eggplant Cultivar‘Yuqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 77-78.
[6]	LIN Yuanmi, ZHU Wenjiao, CHEN Min, XUE Chunmei, JIN Fangyu, ZHU Yuping, JIANG Xinyue, YE Lingfeng, NI Shunanling, YANG Qing. Mir396b Negatively Regulates Eggplant Defense Response to Verticillium Wilt [J]. Acta Horticulturae Sinica, 2022, 49(8): 1713-1722.
[7]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.
[8]	QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool [J]. Acta Horticulturae Sinica, 2022, 49(3): 613-621.
[9]	LIN Hui, XUE Zhuzheng, HUANG Jiandu, CHEN Jibing, WEN Qingfang. A New Eggplant Cultivar‘Fuqie 8’ [J]. Acta Horticulturae Sinica, 2022, 49(2): 463-464.
[10]	QIAO Jun, WANG Liying, LIU Jing, LI Suweng. Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing [J]. Acta Horticulturae Sinica, 2022, 49(11): 2347-2356.
[11]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, and GONG Chao. A New Eggplant Cultivar'Xinfeng 2' [J]. Acta Horticulturae Sinica, 2022, 49(10): 2289-2290.
[12]	JIA Li, JIANG Haikun, WANG Yan, YAN Congsheng, WANG Mingxia, DONG Yanxiang, FANG Ling, ZHANG Qi’an, YU Feifei, and GE Zhihuan. A New White Eggplant Cultivar‘Wanqie 048’ [J]. Acta Horticulturae Sinica, 2021, 48(S2): 2837-2838.
[13]	XIE Kaidong, FANG Yanni, WU Xiaomeng, XIE Zongzhou, DENG Xiuxin, and GUO Wenwu. A New Seedless Pummelo Cultivar‘Huayou 2’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2945-2946.
[14]	ZHU Zongwen, WU Xuexia, WANG Yong, JIANG Jun, SHEN Haibin, TIAN Shoubo, and ZHA Dingshi, . A New Eggplant Cultivar‘Lütianshi’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2967-2968.
[15]	TAN Jie, HUANG Shuping, LI Ye, CHEN Xia, ZHANG Hongyuan, CHEN Feng, YU Cailiang, and ZHANG Min, . A New Eggplant Cultivar‘Yingchun 4’ [J]. Acta Horticulturae Sinica, 2020, 47(S2): 2969-2970.

Using Protoplast Electrofusion Technology to Obtain Interspecific SomaticFusion Hybrid of Eggplant

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments