Studies on the Isolation, Culture of Protoplast and Plant Regeration inLuotiantianshi Persimmon

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (4): 369-371.

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Studies on the Isolation, Culture of Protoplast and Plant Regeration inLuotiantianshi Persimmon

Gu Xiaofeng^¹ and Luo Zhengrong^{^1,2*}

(¹College of Horticulture and Forestry , Huaz hong Agricultural University , Wuhan 430070, China; ²Department of Horticulture, Hubei Institute for Nationalities, Enshi 445000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

Abstract

Abstract: The conditions of isolation and culture of protoplasts from leaf primordial excised from dormant buds of adult nonastringent presimmon Luot iantianshi were studied. The best combination of enzymes selected for protoplast isolation from the calli subcultured 10 d was CPW+ 0. 5% Cellulas R-10+ 0. 03% Pedinase+0. 7 mol/ L Mannitol+ 1% PVP-10. Protoplast purification could be obtained by means of floating collection and centrifugat ion. Protoplast divided for the f irst time after 5- 6 d, formed multicell clusters and grew into visible minicallus after 60- 70 d, then minicallus was transferred onto calli agar medium for proliferat ion. When the calli grew to more than 5 mm in diameter, they were induced to advent itious bud and rooted.

Key words: Nonastringent persimmon, Protoplast, Isolation, Culture, Plant regeneration

CLC Number:

S 665. 2

Gu Xiaofeng and Luo Zhengrong;. Studies on the Isolation, Culture of Protoplast and Plant Regeration inLuotiantianshi Persimmon[J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 369-371.

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Studies on the Isolation, Culture of Protoplast and Plant Regeration inLuotiantianshi Persimmon

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