In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (2): 181-182.

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In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce

Zhu Luying^{1 ,3}, Liu Ling² , Meng Xiangdong¹ , and Zhang Zhenxian¹

(¹Department of Horticulture , Shandong Agriculture University , Tai’an 271018 , China ;　²Beijing Vegetable Research Center , Beijing 100089 , China ;　³Department of Biology , Yantai Normal University , Yantai 264025 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

Abstract

Abstract: An efficient and fast method for shoot differentiation from cotyledon explants of lettuce (Lactuca sativa L. ) was developed. Shoot differentiation rate of lettuce cultivars reached as high as 100 % , each explant produced up to 5 - 6 seedlings. Factors influencing in vitro explant differentiation were studied. The shoot differentiation and enlongation rate were improved by using cotyledon of three days as explant . The regeneration period from lettuce cotyledon explants to a seedling was shortened to 30 - 35 days.

Key words: Lactuca sativa L., Tissue culture, Organogensis

CLC Number:

S 636. 2

Zhu Luying;;Liu Ling;Meng Xiangdong;and Zhang Zhenxian. In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce[J]. ACTA HORTICULTURAE SINICA, 2002, 29(2): 181-182.

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In Vitro Shoot Differentiation from Cotyledon Explants of Lettuce

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