Polyploid Induction and Identification of Rhododendron farrerae

doi:10.16420/j.issn.0513-353x.2025-0493

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (9): 2669-2678.doi: 10.16420/j.issn.0513-353x.2025-0493

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Polyploid Induction and Identification of Rhododendron farrerae

SUN Yuwen, LIU Xiuqun^*()

National Key Laboratory of Germplasm Innovation and Utilization of Horticultural Crops， College of Horticulture and Forestry Sciences，Huazhong Agricultural University， Wuhan 430070， China

Received:2026-03-05 Revised:2026-06-03 Online:2026-09-25 Published:2026-09-20
Contact: LIU Xiuqun

Abstract

Abstract:

Polyploid induction of shoot tip and callus of tissue culture seedlings of Rhododendron farrerae by colchicine was carried out. The results showed that the treatment of colchicine concentration of 2.0 g · L^-1 for 24 h was the best combination for inducing stem tip doubling，and the induction rate reached 16.67%. The treatment of colchicine concentration 0.5 g · L^-1 for 24 h was the best combination for inducing callus doubling，and the induction rate was 6.67%. Ploidy identification was performed on the surviving plants after induction. It was observed that the tetraploid plants were shorter and smaller as a whole，the leaves of the tetraploid plants became larger and thicker and showed significant differences in color，the stomatal density was significantly lower than that of the diploid，and the guard cells were significantly increased. The leaf relative water content，chlorophyll content，anthocyanin content and POD activity of tetraploid plants were significantly higher than those of diploid plants.

Key words: Rhododendron farrerae, chromosomal doubling, callus, colchicine, polyploid identification

CLC Number:

S685.21

SUN Yuwen, LIU Xiuqun. Polyploid Induction and Identification of Rhododendron farrerae[J]. Acta Horticulturae Sinica, 2026, 53(9): 2669-2678.

Figures/Tables 10

References 35

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编号 Number	处理浓度/（g · L^-1） Treatment concentration	处理时间/h Treatment time	成活率/% Survival	诱导率/% Inducibility rate
1	0	24	100.00 ± 0.00 a	0 b
2	0	48	100.00 ± 0.00 a	0 b
3	0.5	24	100.00 ± 0.00 a	10.00 ± 10.00 ab
4	0.5	48	93.33 ± 11.55 a	6.67 ± 5.77 ab
5	1.0	24	96.67 ± 5.77 a	3.33 ± 5.77 b
6	1.0	48	86.67 ± 15.28 ab	0 b
7	2.0	24	96.67 ± 5.77 a	16.67 ± 15.28 a
8	2.0	48	70.00 ± 26.46 b	0 b

编号 Number	处理浓度/（g · L^-1） Treatment concentration	处理时间/h Treatment time	成活率/% Survival	诱导率/% Inducibility rate
1	0	24	100.00 ± 0.00 a	0 b
2	0	48	100.00 ± 0.00 a	0 b
3	0.5	24	100.00 ± 0.00 a	10.00 ± 10.00 ab
4	0.5	48	93.33 ± 11.55 a	6.67 ± 5.77 ab
5	1.0	24	96.67 ± 5.77 a	3.33 ± 5.77 b
6	1.0	48	86.67 ± 15.28 ab	0 b
7	2.0	24	96.67 ± 5.77 a	16.67 ± 15.28 a
8	2.0	48	70.00 ± 26.46 b	0 b

编号	处理浓度/（g ·L^-1）	处理时间/h	成活率/%	诱导率/%
Number	Treatment concentration	Treatment time	Survival	Inducibility rate
1	0	24	100.00 ± 0.00 a	0 a
2	0	48	100.00 ± 0.00 a	0 a
3	0.25	24	100.00 ± 0.00 a	0 a
4	0.25	48	86.67 ± 23.09 a	0 a
5	0.50	24	93.33 ± 11.55 a	6.67 ± 11.55 a
6	0.50	48	96.67 ± 5.77 a	3.33 ± 5.77 a
7	1.00	24	93.33 ± 5.77 a	0 a
8	1.00	48	100.00 ± 0.00 a	0 a

编号	处理浓度/（g ·L^-1）	处理时间/h	成活率/%	诱导率/%
Number	Treatment concentration	Treatment time	Survival	Inducibility rate
1	0	24	100.00 ± 0.00 a	0 a
2	0	48	100.00 ± 0.00 a	0 a
3	0.25	24	100.00 ± 0.00 a	0 a
4	0.25	48	86.67 ± 23.09 a	0 a
5	0.50	24	93.33 ± 11.55 a	6.67 ± 11.55 a
6	0.50	48	96.67 ± 5.77 a	3.33 ± 5.77 a
7	1.00	24	93.33 ± 5.77 a	0 a
8	1.00	48	100.00 ± 0.00 a	0 a

倍性 Ploidy	气孔密度/（个 · mm^-2） Stoma density	保卫细胞长/μm Guard cell length	保卫细胞宽/μm Guard cell width
二倍体 Diploid	613.40 ± 5.59	16.18 ± 1.12	14.12 ± 0.83
四倍体 Tetraploid	286.60 ± 1.95^**	24.60 ± 2.77^**	21.50 ± 1.84^**

Polyploid Induction and Identification of Rhododendron farrerae

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倍性 Ploidy	叶片鲜质量/g Leaf fresh weight	叶片饱和鲜质量/g Saturated fresh weight of leaves	叶片干质量/g Leaf dry weight	叶片相对含水量/% Leaf relative water content
二倍体 Diploid	0.0121 ± 0.0003	0.0149 ± 0.0002	0.0044 ± 0.0003	72.9600 ± 0.0087
四倍体 Tetraploid	0.0141 ± 0.0006^**	0.0168 ± 0.0005^**	0.0051 ± 0.0002^*	77.6000 ± 0.0238^*

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