Functional Analysis of AcRhoGAP in Kiwifruit in Response to Psa Infection

doi:10.16420/j.issn.0513-353x.2024-0179

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 25-36.doi: 10.16420/j.issn.0513-353x.2024-0179

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Functional Analysis of AcRhoGAP in Kiwifruit in Response to Psa Infection

SU Yanshan, YANG Yiting, YANG Xiaoya, TANG Rongxia, XI Dehui^*()

Key Laboratory of Bio-resource and Eco-environment of Ministry of Education，College of Life Sciences，Sichuan University，Chengdu 610065，China

Received:2024-10-21 Revised:2024-11-25 Online:2025-01-25 Published:2025-01-18
Contact: XI Dehui

Abstract

Abstract:

As one of the regulatory factors of small G proteins，Rho GTPase-activating protein（RhoGAP）have been reported to play important roles in plant growth and development，cytoskeleton regulation，and signal transduction. However，their function in plant disease resistance response is still largely unknown. This article explores the role of AcRhoGAP（A RhoGAP gene in Actinidia chinensis Planch.）in plant response to Psa（Pseudomonas syringae pv. actinidiae）infection using A. chinensis and Nicotiana benthamiana as materials and Agrobacterium mediated transient overexpression method. The results showed that Psa infection induced up-regulation of AcRhoGAP expression in kiwifruit leaves，and leaves with transient overexpression of AcRhoGAP were more susceptible to Psa infection，resulting in more severe damage to the membrane system. In N. benthamiana，the CFU of Psa in AcRhoGAP transient overexpression leaves is higher，the content of reactive oxygen species is reduced，the membrane system is more severely damaged，and the expression of defense related hormone pathway marker genes changes. Taken together，AcRhoGAP may be a negatively factor in the response of kiwifruit to Psa infection.

Key words: Actinidia chinensis, AcRhoGAP, bacterial canker, Pseudomonas syringae pv. actinidiae, pathogenic factor

SU Yanshan, YANG Yiting, YANG Xiaoya, TANG Rongxia, XI Dehui. Functional Analysis of AcRhoGAP in Kiwifruit in Response to Psa Infection[J]. Acta Horticulturae Sinica, 2025, 52(1): 25-36.

Figures/Tables 6

Table 1 Primer sequences used in this study

用途 Use	GI/基因ID号 GI/Gene ID	片段大小/bp Product size	名称 Name	引物序列（5′-3′） Primer sequence
基因克隆Gene cloning	1371493451	1 410	SalⅠ-1307AcGAP-F	GCGTCGACATGACAGAGGTCCTCCACTC
基因克隆Gene cloning	1371493451		SpeⅠ-1307AcGAP-R	GGACTAGTTCACCGCCAGGCTTCA
实时荧光定量 PCR Quantitative real-time PCR	107826390	270	EF1α-F	TGTTGTATGATTTTGGGGTAGAC
	107826390		EF1α-R	GAATCATAAACAGATCATATATGCAC
	1371492652	270	Ac-actinB-F	CAGCATAACGGTCAAGGTC
	1371492652		Ac-actinB-R	GAAAACAAATCTAGAACGAAAA
	1371493451	185	AcGAP-F	CGGGTGGCCGACTAATG
	1371493451		AcGAP-R	GCACACTGTTCCCTCTAGG
	21552982	200	NtEDS1-F	TCTTACCGATATTCCCTTGT
	21552982		NtEDS1-R	CTGCTTCTCCCATTCTCC
	19939	200	NtPR1a-F	ATTGCCTTCATTTCTTCTTG
	19939		NtPR1a-R	TTGGGACACCAGGAGCAT
	107807832	200	NtPR1b-F	GTGGACACTATACTCAGGTG
	107807832		NtPR1b-R	TCCAACTTGGAATCAAAGGG
	107831756	200	NtNPR1-F	ATGCGGATGACTTGTCTG
	107831756		NtNPR1-R	GGGCTTATCTACTCCCTTA
	107770253	200	NtLOX-F	GGTCAAGAAGTTGTGAACATACA
	107770253		NtLOX-R	TTTGTCCAGCATCTCTGCA
	107783985	200	NtAOS-F	TCCAGTCTTACCACTACATAAAA
	107783985		NtAOS-R	AAGAGAATGGAAAGGAAGTG
	109215681	200	NtPDF1.2-F	AGATGGGACCAACGACAA
	109215681		NtPDF1.2-R	AAATCCTTCGGTCAAACA
	107819687	200	NtCOI1-F	AGCAGCCCATTGTTTCTT
	107819687		NtCOI1-R	CACCTTGTTCATCCTCCA
	109235543	200	NtEIN2-F	TCATCATCAATGTCGTCAAC
	109235543		NtEIN2-R	GCTAAGGTCGGTAATAGTGT
半定量PCR Semi quantitative PCR	107826390	270	EF1α-F	TGTTGTATGATTTTGGGGTAGAC
	107826390		EF1α-R	GAATCATAAACAGATCATATATGCAC
	1371492652	270	Ac-actinB-F	CAGCATAACGGTCAAGGTC
	1371492652		Ac-actinB-R	GAAAACAAATCTAGAACGAAAA
	39515390	280	Psa-F1	TTTTGCTTTGCACACCCGATTTT
	39515390		Psa-R2	CACGCACCCTTCAATCAGGATG

Fig. 1 Temporal and spatial expression of AcRhoGAP in Hongyang（A- D）and Hayward（E-H）kiwifruit leaves after Psa infection A，E：The phenotypes after inoculation of Psa；B，F：Psa CFU detected by tablet counting；C，G：Psa CFU detected by semi quantitative PCR；D，H：Relative expression level of AcRhoGAP. ** indicates the difference between two materials reaches a significant level when P < 0.01

Fig. 2 Effects of instantaneous overexpression of AcRhoGAP on Psa infection in Hongyang kiwifruit leaves A：The effect of transient overexpression；B：The phenotypes of disease progression；C：Psa CFU detected by tablet counting；D：Psa CFU detected by semi quantitative PCR；E：Relative EL；F：MDA content. ***，**，* respectively indicate the difference between two materials reaches a significant level when P < 0.001，P < 0.01，P < 0.05

Fig. 3 Effects of instantaneous overexpression of AcRhoGAP on Psa infection in Nicotiana benthamiana leaves A：The effect of transient overexpression；B：The phenotypes of disease progression；C：Psa CFU detected by tablet counting；D：Psa CFU detected by semi quantitative PCR. ***，** respectively indicate the difference between two materials reaches a significant level when P < 0.001，P < 0.01

Fig. 4 Effects of Psa infection on reactive oxygen species production and membrane system damage after transient overexpression of AcRhoGAP in Nicotiana benthamiana leaves A：Superoxide anion content detected by NBT staining method；B：H2O2 content detected by DAB staining method；C：Relative EL；D：MDA content. **，* respectively indicate the difference between two materials reaches a significant level when P < 0.01，P < 0.05

Fig. 5 Effects of instantaneous overexpression of AcRhoGAP on the expression of the marker gene in the anti disease signaling pathway in Nicotiana benthamiana leaves ***，**，* respectively indicate the difference between two materials reaches a significant level when P < 0.001，P < 0.01，P < 0.05 .

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Functional Analysis of AcRhoGAP in Kiwifruit in Response to Psa Infection

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