Isolation and Identification of Endophytic Fungus Pa2 Antagonistic Against Apple Ring Rot Caused by Botryosphaeria dothidea

doi:10.16420/j.issn.0513-353x.2023-0292

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 1113-1125.doi: 10.16420/j.issn.0513-353x.2023-0292

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Isolation and Identification of Endophytic Fungus Pa2 Antagonistic Against Apple Ring Rot Caused by Botryosphaeria dothidea

SHI Bingke¹, WANG Zhuoni¹, QIN Genhong¹, HUANG Tianxiang¹, WANG Li¹, TU Hongtao¹^,³, YUAN Hongbo¹^,²^,^*(), HOU Hui¹^,³^,^*()

¹ Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China
² Western Agricultural Research Center，Chinese Academy of Agricultural Sciences，Changji，XinJiang 831100，China
³ Zhongyuan Research Center，Chinese Academy of Agricultural Sciences，Xinxiang，Henan 453004，China

Received:2023-12-11 Revised:2024-03-28 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

In order to isolate and screen antagonistic fungi against apple ring rot caused by Botryosphaeria dothidea，the present study isolated endophytic fungi from the branches of apple ring rot resistant cultivar by tissue isolation methods. The results showed that five antagonistic fungal strains were obtained from 40 isolated strains，among which strain Pa2，identified as Fusarium lateritium，had the best inhibitory effect on B. dothidea with 77.49% inhibition rate. Strain Pa2 supernatant treatment can not only destroy the hyphae cell membrane，but also induce pathogenesis-related gene（MdPR1）expression in apple fruit. The result showed that the control effect of strain Pa2 on apple ring rot was 63.63% at 5 dpi.

Key words: apple ring rot, antagonistic fungus, Fusarium lateritium, control effect

SHI Bingke, WANG Zhuoni, QIN Genhong, HUANG Tianxiang, WANG Li, TU Hongtao, YUAN Hongbo, HOU Hui. Isolation and Identification of Endophytic Fungus Pa2 Antagonistic Against Apple Ring Rot Caused by Botryosphaeria dothidea[J]. Acta Horticulturae Sinica, 2024, 51(5): 1113-1125.

Figures/Tables 14

References 38

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基因 Gene	引物 Primer	引物序列（5′-3′） Primer sequence
ITS	ITS1	TCCGTAGGTGAACCTGCGG
	ITS4	TCCTCCGCTTATTGATATGC
Tubulin	Bt2a	GGTAACCAAATCGGTGCTGCTTTC
	Bt2b	ACCCTCAGTGTAGTGACCCTTGGC
MdEF1α	MdEF1α-q_F	ACATTGCCCTGTGGAAGTT
	MdEF1α-q_R	GTCTGACCATCCTTGGAAA
MdPR1	MdPR1-q_F	GCAGCAGTAGGCGTTGGTCCCT
MdPR1	MdPR1-q_R	CCAGTGCTCATGGCAAGGTTTT

基因 Gene	引物 Primer	引物序列（5′-3′） Primer sequence
ITS	ITS1	TCCGTAGGTGAACCTGCGG
	ITS4	TCCTCCGCTTATTGATATGC
Tubulin	Bt2a	GGTAACCAAATCGGTGCTGCTTTC
	Bt2b	ACCCTCAGTGTAGTGACCCTTGGC
MdEF1α	MdEF1α-q_F	ACATTGCCCTGTGGAAGTT
	MdEF1α-q_R	GTCTGACCATCCTTGGAAA
MdPR1	MdPR1-q_F	GCAGCAGTAGGCGTTGGTCCCT
MdPR1	MdPR1-q_R	CCAGTGCTCATGGCAAGGTTTT

病原真菌 Pathogenic fungi	抑制率/% Inhibition
苹果轮纹病菌 Botryosphaeria dothidea	77.49 ± 0.92
苹果腐烂病菌 Valsa mali	75.34 ± 1.14
苹果炭疽病菌 Colletotrichum gloeosporioides	74.50 ± 0.39
梨树腐烂病菌 Valsa pyri	74.65 ± 2.11

病原真菌 Pathogenic fungi	抑制率/% Inhibition
苹果轮纹病菌 Botryosphaeria dothidea	77.49 ± 0.92
苹果腐烂病菌 Valsa mali	75.34 ± 1.14
苹果炭疽病菌 Colletotrichum gloeosporioides	74.50 ± 0.39
梨树腐烂病菌 Valsa pyri	74.65 ± 2.11

处理 Treatment	平均病斑长度/cm Lesion length		发病率/% Disease incidence		防治效果/% Control effect
处理 Treatment	3 d	5 d	3 d	5 d	3 d	5 d
对照Control	2.19 ± 0.04 a	3.58 ± 0.06 a	100.00 ± 0.00 a	100.00 ± 0.00 a	—	—
Pa2孢子悬浮液Pa2 spore suspension	0.68 ± 0.00 b	1.30 ± 0.06 b	76.67 ± 4.71 b	100.00 ± 0.00 a	68.95 ± 0.66 b	63.69 ± 1.90 b
多菌灵Carbendazim	0 ± 0.00 c	0 ± 0.00 c	0 ± 0.00 c	0 ± 0.00 b	100.00 ± 0.00 a	100.00 ± 0.00 a

Isolation and Identification of Endophytic Fungus Pa2 Antagonistic Against Apple Ring Rot Caused by Botryosphaeria dothidea

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References 38

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处理 Treatment	平均病斑长度/cm Lesion length		发病率/% Disease incidence		防治效果/% Control effect
处理 Treatment	3 d	5 d	3 d	5 d	3 d	5 d
对照Control	2.10 ± 0.02 a	3.32 ± 0.14 a	100.00 ± 0.00 a	100.00 ± 0.00 a	—	—
Pa2孢子悬浮液 Pa2 spore suspension	0.78 ± 0.03 b	0.97 ± 0.05 b	86.67 ± 4.00 b	90.00 ± 0.00 b	62.90 ± 1.92 b	70.78 ± 3.00 b
多菌灵Carbendazim	2.12 ± 0.02 a	3.29 ± 0.06 a	100.00 ± 0.00 a	100.00 ± 0.00 a	0 ± 0.00 c	0 ± 0.00 c
戊唑醇 Tebuconzole	0 ± 0.00 c	0 ± 0.00 c	0 ± 0.00 c	0 ± 0.00 c	100.00 ± 0.00 a	100.00 ± 0.00 a

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