The Characteristics and Chloroplast Ultrastructure of Yellow Leaf Mutant chl234 in Eggplant

doi:10.16420/j.issn.0513-353x.2022-0300

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (5): 1000-1008.doi: 10.16420/j.issn.0513-353x.2022-0300

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

The Characteristics and Chloroplast Ultrastructure of Yellow Leaf Mutant chl234 in Eggplant

LI Shupei^,^*, ZHANG Ying^,^*, SHU Jinshuai, CHEN Yuhui, YANG Jinkun, CHEN Lulu, LIU Fuzhong()

State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2022-06-02 Revised:2023-01-16 Online:2023-05-25 Published:2023-05-31
Contact: LIU Fuzhong E-mail:lfzcaas@126.com

Abstract

Abstract:

Yellow leaf eggplant mutant chl234 and its wild type were used as materials. chl234 exhibited a yellow-leaf phenotype from cotyledon stage and the true leaves exhibited yellow existed throughout the life cycle. Compared with wild type，chl234 had smaller cotyledon and delayed flowering，and there was no significant difference in the first flower node，plant growth trend，fruit size and seed size between chl234 and wild type. The chlorophyll content in chl234 leaves was significantly lower than that in wild type at the seeding stage，first flowering stage and fourth flowering stage. The net photosynthetic rate，stomatal conductance and transpiration rate of chl234 were significantly lower than that of wild type at the seedling stage，while the intercellular CO₂ concentration was significantly higher than wild type. The net photosynthetic rate，transpiration rate and intercellular CO₂ concentration was no significant difference between chl234 and wild type，while stomatal conductance was significantly lower than that of wild type at fourth flowering stage. chl234 had less grana lamellae stacked pile and irregular arrangement of base grains. The inheritance of the leaf color yellowing trait of chl234 using six genetic analysis populations showed that the leaf color yellowing trait was controlled by a recessive nuclear single gene.

Key words: eggplant, yellow leaf mutant, chlorophyll content, photosynthetic properties, chloroplast ultrastructure, genetic analysis

CLC Number:

S641.1

LI Shupei, ZHANG Ying, SHU Jinshuai, CHEN Yuhui, YANG Jinkun, CHEN Lulu, LIU Fuzhong. The Characteristics and Chloroplast Ultrastructure of Yellow Leaf Mutant chl234 in Eggplant[J]. Acta Horticulturae Sinica, 2023, 50(5): 1000-1008.

Figures/Tables 6

References 28

[1]	Carol P, Stevenson D, Bisanz C, Breitenbach J, Sandmann G, Mache R, Coupland G, Kuntz M. 1999. Mutations in the arabidopsis gene IMMUTANS cause a variegated phenotype by inactivating a chloroplast terminal oxidase associated with phytoene desaturation. Plant Cell, 11 (1):57-68. pmid: 9878632
[2]	Cui Li-peng, Song Li-hua, Huang Ze-jun, Gao Jian-chang, Guo Yan-mei, Du Yong-chen, Wang Xiao-xuan. 2017. Physiological characteristics and genetic mapping of tomato yellow leaf gene Netted Viresce(NV). China Vegetables,(7):29-36. (in Chinese)
	崔丽朋, 宋丽华, 黄泽军, 高建昌, 国艳梅, 杜永臣, 王孝宣. 2017. 番茄黄化基因Netted Viresce(NV)的遗传定位及生理特性研究. 中国蔬菜,(7):29-36.
[3]	Dong Zun, Liu Jing-yang, Ma Hong-mei, Xu Cai-kang, Sun Hua, Zhang Jian-dong. 2000. Chlorophyll contents and chloroplast ultrastructure of chlorophyll deficient mutant in B.napus. Chinese Journal of Oil Crop Sciences,(3):28-30. (in Chinese)
	董遵, 刘敬阳, 马红梅, 许才康, 孙华, 张建栋. 2000. 甘蓝型油菜黄化(苗)突变体的叶绿素含量及超微结构. 中国油料作物学报,(3):28-30.
[4]	Falbel T G, Meehl J B, Staehelin L A. 1996. Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light lntensity and the severity of the block in chlorophyll synthesis. Plant Physiology, 112 (2):821-832. doi: 10.1104/pp.112.2.821 pmid: 8883392
[5]	Guo Yan-mei, Gu Xing-fang, Zhang Chun-zhen, Fang Xiu-juan, Zhang Sheng-ping, Xu Cai-qing. 2003. Genetic mechanism of the cucumber leaf mutant. Acta Horticulturae Sinica, 30 (4):409-412. (in Chinese)
	国艳梅, 顾兴芳, 张春震, 方秀娟, 张圣平, 徐彩清. 2003. 黄瓜叶色突变体遗传机制的研究. 园艺学报, 30 (4):409-412.
[6]	Hu Liang-liang, Zhao Zi-yao, Zhang Hai-qiang, Chen Fei-fan, Zhang Chao-wen, Rong Fu-xi, Chen Peng, Li Yu-hong. 2018. Photosyntheic characteristics analysis of new leaf color mutant in cucumber. Acta Agriculturae Boreali-occidentalis Sinica, 27 (11):1622-1628. (in Chinese)
	胡亮亮, 赵子瑶, 张海强, 陈菲帆, 张朝文, 戎福喜, 陈鹏, 李玉红. 2018. 一个新的黄瓜叶色突变体的光合特性分析. 西北农业学报, 27 (11):1622-1628.
[7]	Ji Xian-jun, Ye Sheng-hai, Zhou Ya, Xiu Fen-lian, Deng Xiao-mei, Shang Hai-xuan, Liu Ji-yun, Chen Ping-ping, Jin Qing-sheng, Zhang Xiao-ming. 2012. Genetic analysis and mapping of three rice mutants with yellow leaf trait. Acta Agriculturae Zhejiangensis, 24 (1):7-11. (in Chinese)
	纪现军, 叶胜海, 周涯, 修芬连, 邓晓梅, 尚海漩, 刘继云, 陈萍萍, 金庆生, 张小明. 2012. 3个水稻叶色突变体的遗传分析及基因定位. 浙江农业学报, 24 (1):7-11.
[8]	Jung K H, Hur J, Ryu C H, Choi Y, Chung Y Y, Miyao A, Hirochika H, An G. 2003. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 44 (5):463-472. doi: 10.1093/pcp/pcg064 URL
[9]	Kong Ke-ke, Xu Meng-ge, Wang Ya-qi, Kong Jie-jie, Al-Amin G M, Zhao Tuan-jie. 2017. Gene mapping and character performance of a yellow-green leaf mutant NJ9903-5 in soybean. Soybean Science, 36 (4):494-501. (in Chinese)
	孔可可, 许孟歌, 王亚琪, 孔杰杰, Al-Amin G M, 赵团结. 2017. 大豆黄绿叶突变体NJ9903-5性状表现与基因定位研究. 大豆科学, 36 (4):494-501.
[10]	Li C, Ma F R, Jiao R J, Chen C P, Wang Q, Xiao F L, Sun C H, Deng X J, Dong C L, Wang P R. 2019. Mutation in mg-protoporphyrin IX monomethyl ester cyclase causes yellow and spotted leaf phenotype in rice. Plant Molecular Biology Reporter, 37 (4):253-264. doi: 10.1007/s11105-019-01152-7
[11]	Li Xi-xiang, Zhu De-wei. 2006. Descriptors and data standard for eggplant(Solanum melongena L.). Beijing: China Agriculture Press. (in Chinese)
	李锡香, 朱德蔚. 2006. 茄子种质资源描述规范和数据标准. 北京: 中国农业出版社.
[12]	Lichtenthaler H K, Kuhn G, Prenzel U, Buschmann C, Meier D. 1982. Adaptation of chloroplast-ultrastructure and of chlorophyll- protein levels to high-light and low-light growth conditions. Zeitschrift für Naturforschung C, 37 (5):464-475. doi: 10.1515/znc-1982-5-619 URL
[13]	Liu Fuzhong, Zhang Ying, Yang Jinkun, Chen Yuhui, Shu Jinshuai, Li Shupei, Chen Lulu. 2020. Characterization and genetic analysis of a yellowing mutant of eggplant leaf color. Acta Horticulturae Sinica, 47 (12):2340-2348. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0829
	刘富中, 张映, 杨锦坤, 陈钰辉, 舒金帅, 李淑培, 陈露露. 2020. 茄子叶色黄化突变体的特征及遗传分析. 园艺学报, 47 (12):2340-2348.
[14]	Liu X P, Yang C, Han F Q, Fang Z Y, Yang L M, Zhuang M, Lv H H, Liu Y M, Li Z S, Zhang Y Y. 2016. Genetics and fine mapping of a yellow-green leaf gene(ygl-1)in cabbage(Brassica oleracea var. capitata L.). Molecular Breeding, 36 (6):82. doi: 10.1007/s11032-016-0509-4 URL
[15]	Liu X P, Yu H L, Han F Q, Li Z Y, Fang Z Y, Yang L M, Zhuang M, Lv H H, Liu Y M, Li Z S, Li X, Zhang Y Y. 2018. Differentially expressed genes associated with the cabbage yellow-green-leaf mutant in the ygl-1 mapping interval with recombination suppression. International Journal of Molecular Sciences, 19 (10):2936. doi: 10.3390/ijms19102936 URL
[16]	Lonosky P M, Zhang X, Honavar V G, Dobbs D L, Fu A, Rodermel S R. 2004. A proteomic analysis of maize chloroplast biogenesis. Plant Physiology, 134 (2):560-574. pmid: 14966246
[17]	Miao Han, Gu Xing-fang, Zhang Sheng-ping, Fang Zhi-yuan, Zhang Zhen-xian. 2010. Comparison and analysis of two yellow-green leaf mutants in cucumber(Cucumis sativus L.). China Vegetables,(22):16-20. (in Chinese)
	苗晗, 顾兴芳, 张圣平, 方智远, 张振贤. 2010. 两个黄瓜(Cucumis sativus L.)叶色突变体的比较分析研究. 中国蔬菜,(22):16-20.
[18]	Shao Qin, Yu Ze-yuan, Li Xing-guo, Li Wei, Gao Yan-juan. 2013. Studies on internal physiological and biochemical changes of xantha mutant in melon leaves. China Vegetables,(14):59-65. (in Chinese)
	邵勤, 于泽源, 李兴国, 李为, 高艳娟. 2013. 叶色黄化突变体甜瓜叶片内部生理生化变化的研究. 中国蔬菜,(14):59-65.
[19]	Sheng Z H, Lv Y S, Li W, Luo R J, Wei X J, Xie L H, Jiao G A, Shao G N, Wang J L, Tang S Q, Hu P S. 2017. Yellow-Leaf 1 encodes a magnesium-protoporphyrin IX monomethyl ester cyclase,involved in chlorophyll biosynthesis in rice(Oryza sativa L.). PLoS ONE, 12 (5):e177989.
[20]	Shu Zhan, Zhang Xiao-su, Chen Juan, Chen Gen-yun, Xu Da-quan. 2010. The simplification of chlorophyll content measurement. Plant Physiology Communication, 46 (4):399-402. (in Chinese)
	舒展, 张晓素, 陈娟, 陈根云, 许大全. 2010. 叶绿素含量测定的简化. 植物生理学通讯, 46 (4):399-402.
[21]	Song Ming-mei, Fan Shu-li, Pang Chao-you, Wei Heng-ling, Yu Shu-xun, Song Mei-zhen. 2014. Research on the main photosynthetic characteristics and agronomic traits in virescent cotton materials. Cotton Science, 26 (6):531-538. (in Chinese)
	宋明梅, 范术丽, 庞朝友, 魏恒玲, 喻树迅, 宋美珍. 2014. 棉花芽黄材料主要光合特性和农艺性状的研究. 棉花学报, 26 (6):531-538.
[22]	Tan Xin-xing, Xu Da-quan, Tang Ze-sheng. 2002. Photosynthesis and chlorophyll fluorescence in chlorophyll-deficient barley mutants. Physiology and Molecular Biology of Plants,(1):51-57. (in Chinese)
	谭新星, 许大全, 汤泽生. 1996. 叶绿素缺乏的大麦突变体的光合作用和叶绿素荧光. 植物生理学报,(1):51-57.
[23]	Wang Ya-ling, Li Xiao-han, Gong Fang-lin, Liang Jia-jia, Li Jin-hua. 2019. Phenotypic identification and genetic analysis of a novel tomato yellow green leaf mutant. Journal of Plant Genetic Resources, 20 (1):215-220. (in Chinese) doi: 10.13430/j.cnki.jpgr.20180518001
	王亚玲, 李小寒, 宫方林, 梁佳佳, 李金华. 2019. 一个新的番茄黄绿叶突变体表型鉴定与遗传分析. 植物遗传资源学报, 20 (1):215-220. doi: 10.13430/j.cnki.jpgr.20180518001
[24]	Wang Zu-xiu, Tang Ze-sheng. 1996. Genetic analysis of yellow leaf colour mutant in baley. Southwest China Journal of Agricultural Sciences,(S1):180-182. (in Chinese)
	王祖秀, 汤泽生. 1996. 大麦叶色黄化突变体的遗传分析. 西南农业学报,(S1):180-182.
[25]	Xiao Hua-gui, Yang Huan-wen, Rao Yong, Yang Bin, Zhu Ying, Zhang Wen-long. 2013. Analysis of chloroplast ultrastructure,stomatal characteristic parameters and photosynthetic characteristics of chlorophyllreduced mutant in Brassica napus L. Scientia Agricultura Sinica, 46 (4):715-727. (in Chinese) doi: 10.3864/j.issn.0578-1752.2013.04.006
	肖华贵, 杨焕文, 饶勇, 杨斌, 朱英, 张文龙. 2013. 甘蓝型油菜黄化突变体的叶绿体超微结构、气孔特征参数及光合特性. 中国农业科学, 46 (4):715-727. doi: 10.3864/j.issn.0578-1752.2013.04.006
[26]	Xu Dong-ping, Wang Han-yu, Zhang Cai-bo, Rong Ting-zhao, Cao Mo-ju. 2012. The preliminary study of a novel yellow-green leaf mutant in maize. Journal of Nuclear Agricultural Sciences, 26 (7):988-993. (in Chinese)
	徐冬平, 汪瀚宇, 张采波, 荣廷昭, 曹墨菊. 2012. 一个新的玉米黄化突变体的初步研究. 核农学报, 26 (7):988-993. doi: 10.11869/hnxb.2012.07.0988
[27]	Yang Chong, Zhang Yang-yong, Fang Zhi-yuan, Liu Yu-mei, Yang Li-mei, Zhuang Mu, Sun Pei-tian. 2014. Photosynthetic physiological characteristics and chloroplast ultrastructure of yellow leaf mutant YL-1 in cabbage. Acta Horticulturae Sinica, 41 (6):1133-1144. (in Chinese)
	杨冲, 张扬勇, 方智远, 刘玉梅, 杨丽梅, 庄木, 孙培田. 2014. 甘蓝叶色黄化突变体YL-1的光合生理特性及其叶绿体的超微结构. 园艺学报, 41 (6):1133-1144.
[28]	Zheng Wei, Shi Zheng, Long Mei, Yun-cheng. 2021. Photosynthetic and physiological characteristics analysis of yellow-green leaf mutant in wheat of Jimai5265yg. Scientia Agricultura Sinica, 54 (21):4539-4551. (in Chinese)
	郑伟, 师筝, 龙美, 廖允成. 2021. 黄绿叶突变体冀麦5265yg的光合生理特性分析. 中国农业科学, 54 (21):4539-4551. doi: 10.3864/j.issn.0578-1752.2021.21.005

材料	子叶长/cm	子叶宽/cm	始花期/d	始花节位	叶片长/cm	叶片宽/cm
Material	Cotyledon length	Cotyledon width	First flowering days	First flower node	Leaf length	Leaf width
WT	2.94 ± 0.02 a	1.12 ± 0.04 a	88.54 ± 3.15 a	11.44 ± 0.16 a	24.00 ± 0.27 a	17.22 ± 0.08 a
chl234	2.09 ± 0.09 b	0.86 ± 0.02 b	91.62 ± 4.03 a	11.11 ± 0.16 a	23.56 ± 1.50 a	16.94 ± 0.97 a
材料	株高/cm	株幅/cm	果实纵径/cm	果实横径/cm	单果质量/g	千粒质量/g
Material	Plant height	Plant width	Fruit length	Fruit diameter	Fruit weight	Thousand grains weight
WT	116.67 ± 1.36 a	76.67 ± 4.91 a	27.89 ± 1.44 a	6.19 ± 0.14 a	280.85 ± 6.44 a	4.87 ± 0.24 a
chl234	113.89 ± 0.79 a	85.56 ± 0.79 a	25.11 ± 0.21 a	6.03 ± 0.17 a	232.00 ± 8.14 b	4.13 ± 0.06 b

材料	子叶长/cm	子叶宽/cm	始花期/d	始花节位	叶片长/cm	叶片宽/cm
Material	Cotyledon length	Cotyledon width	First flowering days	First flower node	Leaf length	Leaf width
WT	2.94 ± 0.02 a	1.12 ± 0.04 a	88.54 ± 3.15 a	11.44 ± 0.16 a	24.00 ± 0.27 a	17.22 ± 0.08 a
chl234	2.09 ± 0.09 b	0.86 ± 0.02 b	91.62 ± 4.03 a	11.11 ± 0.16 a	23.56 ± 1.50 a	16.94 ± 0.97 a
材料	株高/cm	株幅/cm	果实纵径/cm	果实横径/cm	单果质量/g	千粒质量/g
Material	Plant height	Plant width	Fruit length	Fruit diameter	Fruit weight	Thousand grains weight
WT	116.67 ± 1.36 a	76.67 ± 4.91 a	27.89 ± 1.44 a	6.19 ± 0.14 a	280.85 ± 6.44 a	4.87 ± 0.24 a
chl234	113.89 ± 0.79 a	85.56 ± 0.79 a	25.11 ± 0.21 a	6.03 ± 0.17 a	232.00 ± 8.14 b	4.13 ± 0.06 b

时期 Period	材料 Material	叶绿素/（µg · mL^-1）Chlorophyll				叶绿素a/b Chlorophyll a/b
时期 Period	材料 Material	a	b	a + b		叶绿素a/b Chlorophyll a/b
苗期Seedling stage	WT	15.34 ± 0.41 a	5.05 ± 0.22 a		20.39 ± 0.62 a	3.04 ± 0.05 b
苗期Seedling stage	chl234	5.77 ± 0.26 b	1.38 ± 0.13 b		7.15 ± 0.39 b	4.19 ± 0.21 a
门茄开花期First flowering stage	WT	22.33 ± 0.33 a	7.33 ± 0.06 a		29.65 ± 0.39 a	3.05 ± 0.03 b
门茄开花期First flowering stage	chl234	8.91 ± 0.28 b	1.81 ± 0.13 b		10.73 ± 0.39 b	5.00 ± 0.24 a
四门斗开花期Fourth flowering stage	WT	23.74 ± 0.04 a	10.02 ± 0.20 a		33.76 ± 0.16 a	2.39 ± 0.05 b
四门斗开花期Fourth flowering stage	chl234	11.81 ± 0.43 b	2.83 ± 0.13 b		14.64 ± 0.43 b	4.18 ± 0.26 a

时期 Period	材料 Material	叶绿素/（µg · mL^-1）Chlorophyll				叶绿素a/b Chlorophyll a/b
时期 Period	材料 Material	a	b	a + b		叶绿素a/b Chlorophyll a/b
苗期Seedling stage	WT	15.34 ± 0.41 a	5.05 ± 0.22 a		20.39 ± 0.62 a	3.04 ± 0.05 b
苗期Seedling stage	chl234	5.77 ± 0.26 b	1.38 ± 0.13 b		7.15 ± 0.39 b	4.19 ± 0.21 a
门茄开花期First flowering stage	WT	22.33 ± 0.33 a	7.33 ± 0.06 a		29.65 ± 0.39 a	3.05 ± 0.03 b
门茄开花期First flowering stage	chl234	8.91 ± 0.28 b	1.81 ± 0.13 b		10.73 ± 0.39 b	5.00 ± 0.24 a
四门斗开花期Fourth flowering stage	WT	23.74 ± 0.04 a	10.02 ± 0.20 a		33.76 ± 0.16 a	2.39 ± 0.05 b
四门斗开花期Fourth flowering stage	chl234	11.81 ± 0.43 b	2.83 ± 0.13 b		14.64 ± 0.43 b	4.18 ± 0.26 a

时期 Period	材料 Material	净光合速率/（μmol · m^-2· s^-1）	气孔导度/ （mol · m^-2 · s^-1）	蒸腾速率/ （mmol · m^-2· s^-1）	胞间CO₂浓度/（μmol · mol^-1）
时期 Period	材料 Material	Net photosynthetic rate	Stomatal conductance	Transpiration rate	Intercellular carbon dioxide
苗期	WT	20.20 ± 0.43 a	321.67 ± 4.11 a	6.90 ± 0.24 a	228.00 ± 6.38 b
Seedling stage	chl234	14.90 ± 1.43 b	232.00 ± 17.72 b	5.23 ± 0.26 b	253.67 ± 3.40 a
四门斗开花期	WT	28.70 ± 2.34 a	728.33 ± 52.49 a	9.23 ± 0.63 a	280.33 ± 42.16 a
Fourth flowering stage	chl234	26.43 ± 0.79 a	430.33 ± 30.57 b	9.17 ± 0.57 a	244.33 ± 11.47 a

The Characteristics and Chloroplast Ultrastructure of Yellow Leaf Mutant chl234 in Eggplant

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 28

Related Articles 15

Recommended Articles

Metrics

Comments

群体	总株数	绿叶株数	黄叶株数	分离比值	理论分离比率
Population	Total number	Number of green	Number of yellow	Segregation ratio	Theoretical ratio
P₁（chl234）	27		27
P₂（WT）	30	30
F_{1（P1 × P2）}	31	31
F_{1（P2 × P1）}	26	26
F₂	676	496	180	2.76:1	3:1
BC₁P₁	201	109	92	1.18:1	1:1
BC₁P₂	30	30			1:0

[1]	WANG Hong and ZHANG Ya. A New Eggplant Cultivar‘Hangqie 2010’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 61-62.
[2]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, GONG Chao. A New Eggplant Cultivar‘Xinfeng 3’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 227-228.
[3]	CAI Peng, FANG Chao, LI Yuejian, LIU Duchen, LIU Xiaojun, LIANG Genyun. A New Eggplant Cultivar‘Tianjiao’ [J]. Acta Horticulturae Sinica, 2023, 50(1): 229-230.
[4]	TAN Jie, HUANG Shuping, CHEN Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 99-100.
[5]	HUANG Shuping, TAN Jie, Chen Xia, ZHANG Hongyuan, LI Ye, WANG Benqi, CHEN Hao, WU Xuexia, and ZHANG Min, . A New Eggplant Cultivar‘Eqie 6’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 101-102.
[6]	YANG Yang, TIAN Shibing, WANG Yongqing, JIANG Changchun, ZOU Min, TAO Tao, ZHOU Shanshan, WANG Zhijin, BAO Zhongxian, and TANG Xiaohua. A New Eggplant Cultivar‘Yuqie 5’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 77-78.
[7]	LIN Yuanmi, ZHU Wenjiao, CHEN Min, XUE Chunmei, JIN Fangyu, ZHU Yuping, JIANG Xinyue, YE Lingfeng, NI Shunanling, YANG Qing. Mir396b Negatively Regulates Eggplant Defense Response to Verticillium Wilt [J]. Acta Horticulturae Sinica, 2022, 49(8): 1713-1722.
[8]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.
[9]	WANG Ying, QIN Yangyang, ZENG Ting, LIAO Ping, ZHANG Wei, ZHOU Yan, ZHOU Changyong. Effects of Citrus Yellow Vein Clearing Virus on Photosynthetic Characteristics and Chloroplast Ultrastructure of Lemon [J]. Acta Horticulturae Sinica, 2022, 49(4): 861-867.
[10]	QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool [J]. Acta Horticulturae Sinica, 2022, 49(3): 613-621.
[11]	TANG Chenqian, QIU Zhixin, TAN Chao, QIAN Yuming, CHEN Xin. Sorbus koehneana（Rosaceae）：Its Complete Chloroplast Genome and Phylogenetic Relationship with S. unguiculata [J]. Acta Horticulturae Sinica, 2022, 49(3): 641-654.
[12]	LIN Hui, XUE Zhuzheng, HUANG Jiandu, CHEN Jibing, WEN Qingfang. A New Eggplant Cultivar‘Fuqie 8’ [J]. Acta Horticulturae Sinica, 2022, 49(2): 463-464.
[13]	QIAO Jun, WANG Liying, LIU Jing, LI Suweng. Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing [J]. Acta Horticulturae Sinica, 2022, 49(11): 2347-2356.
[14]	LI Zhiliang, LI Zhenxing, LI Tao, SUN Baojuan, LI Ying, XU Xiaowan, WANG Hengming, HENG Zhou, and GONG Chao. A New Eggplant Cultivar'Xinfeng 2' [J]. Acta Horticulturae Sinica, 2022, 49(10): 2289-2290.
[15]	FENG Lixiao, HU Rong, BU Shan, ZHANG Deyong, LUO Xiangwen, LI Fan, DING Ming, ZHANG Zhuo, ZHANG Songbai, LIU Yong. Molecular Detection and Genetic Evolution Analysis of Yunnan Isolates of Lettuce Chlorosis Virus in Tomato [J]. Acta Horticulturae Sinica, 2022, 49(1): 141-147.