Classification and Gene Expression Analysis of HSFs from Aquilegia coerulea and Sub-cellular Localization of AyHSF1

doi:10.16420/j.issn.0513-353x.2014-1150

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (8): 1533-1541.doi: 10.16420/j.issn.0513-353x.2014-1150

• Ornamental Plants • Previous Articles Next Articles

Classification and Gene Expression Analysis of HSFs from Aquilegia coerulea and Sub-cellular Localization of AyHSF1

ZHANG Hua-Li, WANG Tao, CUI Rong-Feng, DONG Ai-Xiang, XIN Hai-Bo, YI Ming-Fang

1Beijing Institute of Landscape Architecture，Beijing Key Laboratory of Greening Plants Breeding，Beijing 100102，China；2Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193，China

Online:2015-08-25 Published:2015-08-25

Abstract

Abstract: Fourteen HSFs（Heat shock transcription factors）were identified from Aquilegia coerulea genome sequence. Alignment and phylogenetic analyses displayed there were 8 HSFAs，5 HSFBs and 1 HSFC in A. coerulea. Real-time PCR revealed that transcripts of these HSFs were detected and most of them were induced significantly by heat shock in A. yabeana. Furthermore，HSF1 was cloned from A. yabeana cDNA. The protein fused with GFP at its N-terminus was investigated in onion epidermal cell and observed with a translocation phenomenon.

Key words: Aquilegia coerulea, A. yabeana, heat shock transcription factor（HSF）, heat shock, thermotolerance

CLC Number:

S 682

ZHANG Hua-Li, WANG Tao, CUI Rong-Feng, DONG Ai-Xiang, XIN Hai-Bo, YI Ming-Fang. Classification and Gene Expression Analysis of HSFs from Aquilegia coerulea and Sub-cellular Localization of AyHSF1[J]. ACTA HORTICULTURAE SINICA, 2015, 42(8): 1533-1541.

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Classification and Gene Expression Analysis of HSFs from Aquilegia coerulea and Sub-cellular Localization of AyHSF1

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