番木瓜四倍体与二倍体的核型分析及45S r DNA-FISH研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 345-348.

番木瓜四倍体与二倍体的核型分析及45S r DNA-FISH研究

汪卫星; 李春艳;向素琼;梁国鲁^*

(西南大学园艺园林学院, 重庆400716)

收稿日期:2006-10-13 修回日期:2007-02-13 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 梁国鲁

Karyotypes Analysis and 45S rDNA-FISH of the Tetraploid and Diploid in Carica papaya L.

WANG Wei-xing, LI Chun-yan, XIANG Su-qiong, and LIANG Guo-lu^*

(College of Horticulture and Landscape, Southwest University, Chongqing 400716, China)

Received:2006-10-13 Revised:2007-02-13 Online:2007-04-25 Published:2007-04-25
Contact: LIANG Guo-lu

摘要/Abstract

摘要： 以番木瓜四倍体与二倍体植株为材料, 利用45S rDNA为探针, 结合核型分析与荧光原位杂交
( fluorescence in situ hybridization, 简称FISH) 技术, 分析比较了二者之间的核型特征。结果表明: 二倍体与四倍体番木瓜在核型特征上没有明显差异, 染色体绝对长度在2.35～4.76μm之间, 属于中小染色体,均由中部着丝粒染色体(m) 和近中部着丝粒染色体( sm) 组成, 属“2A”类型, 二倍体核型公式为2n=2x=18=16m (2SAT) +2 sm, 四倍体为2n=4x =36=32m (4SAT) + 4 sm; 在二倍体体细胞中期染色体及间期核中, 均发现两个杂交信号位点, 在四倍体中则发现4个杂交信号位点。二者的杂交信号均位于第6对带有随体的同源染色体的次缢痕区域。由此认为该四倍体是由二倍体直接加倍形成的同源四倍体。

关键词: 番木瓜, 核型, 荧光原位杂交, 同源四倍体

Abstract: By using the techniques of karyotype analysis and fluorescence in situ hybridization with 45SrDNA as p robe, the characteristics of karyotypes and 45S rDNA-FISH of tetrap loid and diploid in Carica papaya L. were studied. The results showed that the karyotypes of tetrap loid and dip loid were similar, the lengths of their chromosomes were 2.35 - 4.76μm as short chromosomes. They were composed of metacentric chromosomes and submetacentric chromosomes, their karyotypeswere 2A, the karyotype formulaswere 2n = 2x =18 = 16m (2SAT) + 2 sm and 2n = 4x = 36 = 32m (4SAT) + 4 sm. Two signals were detected in the karyons and chromosomes of somatic cells of dip loids, and four signalswere detected in that of tetrap loids. These signals were all located in the NucleolarOrganizer Region existed in the sixth pair of homologous chromosomes with satellites. According to the results, it is concluded that tetrap loids are homologous tetrap loidswhich came
into being as the redup lication of chromosomes of somatic cells of diploids.

Key words: Carica papaya L., Karyotype, Fluorescence in situ hybridization, Homologous tetraploid

中图分类号:

S 667.9

汪卫星;李春艳;向素琼;梁国鲁. 番木瓜四倍体与二倍体的核型分析及45S r DNA-FISH研究[J]. 园艺学报, 2007, 34(2): 345-348.

WANG Wei-xing;LI Chun-yan;XIANG Su-qiong;and LIANG Guo-lu. Karyotypes Analysis and 45S rDNA-FISH of the Tetraploid and Diploid in Carica papaya L.[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 345-348.

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