关键词: 大蒜, 紫斑病, 抗性鉴定, 离体接种, 活体接种

Abstract: The infection path of garlic violet leaf spot [Alternaria porri ( ELL. ) Cif. ] and resistance identification of inoculation leaf stages, inoculation spore concentration, inoculation position, culture temperature after inoculation and time to survey etc were studied by using the identification cultivars G087 (R) , G064(MR) , and G073 ( S). The results show that, the pathogen of violet leaf spot infects garlic plants through both the wound and the natural orifices at seedling stage while at flower bud differentiation stage it only invades
through the wound. For disease resistance identification, the op timal leaf stage is 52leaf stage, the optimal spore concentration is 1×10⁶ cfu·mL^-1; Inoculation in vitro ismore simple and rap id compared to inoculation in vivo. For in vitro inoculation, there is no significant difference between leaf face inoculation and leaf back inoculation; The op timal culture temperature after inoculation is 21 ℃; The op timal time to survey disease index is the 6 th day after inoculation. Using G057 (R) , G103 (MR) , and G083 (S) as the verification
cultivars, the results show that the established method is practicable to reflect the true resistance of garlic cultivars to violet leaf spot.

Key words: garlic, violet leaf spot, resistance identification, inoculation in vitro, inoculation in vivo