多毛番茄‘LA2329’对B 型烟粉虱抗性的QTL分析

摘要/Abstract

摘要： 多毛番茄‘LA2329’对烟粉虱具有很高的抗性。利用‘LA2329’与普通番茄‘9706’得到
的BC1 群体构建了一张包含180 个分子标记，总长度为1 166.6 cM 的番茄分子连锁图谱。采用离体鉴定
的方法对BC1 群体进行抗烟粉虱鉴定，鉴定指标为叶片烟粉虱成虫附着量、叶片烟粉虱卵量，叶片正、
背面Ⅵ型腺毛密度。共定位到33 个与4 个指标相关的QTL。其中叶片烟粉虱成虫附着量和卵量显著正相
关，二者定位到8 个相同的QTL，其中2 个主效QTL 定位在2 号染色体，分别介于标记InDel_FT45 ~ SSR57
之间和SSR57 ~ InDel_FT49 之间，解释的表型变异率分别为33.2%和39.8%。叶片背面Ⅵ型腺毛密度相关
的QTL 在6 号染色体具有集群分布的现象，其中4 个显著性QTL 定位在C2_At3g11210 ~ C2_At1g24360
标记之间，解释的总变异率为56%。叶片正面Ⅵ型腺毛密度相关的4 个QTL 和叶片烟粉虱成虫附着量及
卵量相关的4 个QTL 定位在2 号和12 号染色体的相同位点，增效基因来源相反。以上结果说明叶片烟粉
虱成虫附着量和卵量指标具有相同的抗性鉴定效果，可以认为是同一个鉴定指标；此外，番茄中Ⅵ型腺
毛密度不是影响叶片烟粉虱成虫附着量和卵量的性状。

关键词: 多毛番茄, 烟粉虱, 成虫附着量, 卵量, Ⅵ型腺毛密度, QTL

Abstract: The wild species of tomato，Solanum habrochaites LA2329 has high levels of resistance to
whitefly（Bemisia tabaci） and is thus an important resource for breeding of insect resistance. A linkage
map that covered 1 166.6 cM of the tomato genome and included 180 markers was generated based on the
BC1 population of generated from tomato cultivar 9706 crossed with Solanum habrochaites LA2329.
Parents，F1 and BC1 population were bioassayed with whiteflies using an in vitro method. Four
phenotypic traits including number of adult whiteflies per leaflet，number of eggs per leaflet and densities
of type Ⅵ trichomes on adaxial and abaxial surfaces were evaluated. A total of 33 QTLs associated with
the four phenotypic traits were found in the BC1 population. The adult whitefly numbers had a significant
positive relationship with oviposition numbers in the BC1 population. Eight QTLs associated with adult
whitefly numbers and ovipitision numbers were located in the same locus. Of the 8 QTLs，2 major QTLs
were located in the interval defined by the markers InDel_FT45–SSR57 and SSR57–InDel_FT49 on
chromosome 2 and explained 33.2% and 39.8% of phenotypic variation，respectively. A QTL cluster that
associated with density of type Ⅵ trichomes on abaxial surfaces was mapped on chromosome 6. Four
major QTLs affecting density of Ⅵ trichomes in abaxial surfaces were located in the interval defined by
C2_At3g11210–C2_At1g24360 and explained 56% of phenotypic variation. Four QTLs associated with
density of type Ⅵ trichomes on adaxial surfaces were mapped to the same locus as 4 of QTLs associated
with adult whitefly numbers and oviposition numbers，but their additive effects were opposite. The results
showed that adult whitefly numbers and ovipitision numbers were measures of similar phenotypic traits for
resistance to whiteflies. In addition，density of type Ⅵ trichomes on tomato was not the major trait of
tomato to whitefly resistance.

Key words: Solanum habrochaites, Bemisia tabaci, adult whitefly, oviposition number, density of Ⅵ trichome, QTL

中图分类号:

S 641.2

郭广君, 高建昌, 王孝宣, 国艳梅, John C. SNYDER, 杜永臣. 多毛番茄‘LA2329’对B 型烟粉虱抗性的QTL分析[J]. 园艺学报, 2013, 40(4): 663-.

GUO Guang-Jun, GAO Jian-Chang, WANG Xiao-Xuan, GUO Yan-Mei, John C. SNYDER, DU Yong-Chen. QTL Analysis of Resistance to Type B Bemisia tabaci in Solanum habrochaites LA2329[J]. ACTA HORTICULTURAE SINICA, 2013, 40(4): 663-.

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