https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2011, Vol. 38 ›› Issue (1): 101-107.

• 观赏植物 • 上一篇    下一篇

栽培菊与菊属—近缘属杂种远缘杂交的研究

汤访评1,2,陈素梅1,陈发棣1,*,房伟民1   

  1. (1南京农业大学园艺学院,南京 210095;2绍兴文理学院生命科学学院,浙江绍兴 312000)
  • 收稿日期:2010-09-21 修回日期:2010-12-20 出版日期:2011-01-25 发布日期:2011-01-25
  • 通讯作者: 陈发棣

Distant Hybridization Between Chrysanthemum and the Intergeneric Hybrids of Dendranthema and Allied Genera

TANG Fang-ping1,2,CHEN Su-mei1,CHEN Fa-di1,*,and FANG Wei-min1   

  1. (1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2College of Life Science,University of Shaoxing,Shaoxing,Zhejiang 312000,China)
  • Received:2010-09-21 Revised:2010-12-20 Online:2011-01-25 Published:2011-01-25
  • Contact: CHEN Fa-di

摘要: 为利用菊属近缘属优良种质资源,对栽培菊花品种进行改良和种质创新,以菊花品种‘天坠玉露’为母本,大岛野路菊 × 芙蓉菊、南京野菊 × 芙蓉菊和菊花脑 × 菊蒿3个属间杂种为父本进行人工杂交,对杂种后代进行细胞学、形态学观察和基因组双色荧光原位杂交分析。结果发现,以南京野菊 × 芙蓉菊F1为父本进行杂交时,结实率为12.5%,后代中仅检测到‘天坠玉露’和南京野菊两个基因组,芙蓉菊基因组被排斥;以菊花脑 × 菊蒿F1为父本时的杂交结实率为0.25%,后代中也仅检测到‘天坠玉露’和菊花脑两个基因组,菊蒿基因组被排斥;以大岛野路菊 × 芙蓉菊F1为父本时的杂交结实率为22.5%,后代中同时检测到‘天坠玉露’、大岛野路菊和芙蓉菊3个基因组,获得了两属三物种间远缘杂种,其各杂交后代与父母本之间在形态上均存在显著差异。

关键词: 菊花, 菊属, 近缘属, 远缘杂交, 双色荧光原位杂交

Abstract: To innovate chrysanthemum germplasm by introducing elite attributes of its allied genera,artificial hybridizations have been made between Dendranthema morifolium‘Tianzhuiyulu’(2n = 54;♀)and three intergeneric hybrids(♂). The seed set of D. morifolium ‘Tianzhuiyulu’ ×(D. crassum × Crossostephium chinense),D. morifolium‘Tianzhuiyulu’× (D. indicum × C. chinense)and D. morifolium ‘Tianzhuiyulu’×(D. nankingense × Tanacetum vulgare)was 22.5%,12.5% and 0.25% respectively. Dual-color genomic in situ hybridization was applied for these hybrids. In D. morifolium‘Tianzhuiyulu’ × (D. indicum × C. chinense)hybrid,it contained two genomes of D. morifolium‘Tianzhuiyulu’and D. indicum. In D. morifolium‘Tianzhuiyulu’× (D. nankingense × T. vulgare)hybrid,it contained two genomes of D. morifolium‘Tianzhuiyulu’and D. nankingense. While in D. morifolium‘Tianzhuiyulu’×(D. crassum × C. chinense)hybrids,they contained three genomes of D. morifolium‘Tianzhuiyulu’,D. crassum and C. chinense. The morphology of the hybrid and its parents differed significantly from one another.

Key words: Chrysanthemum, Dendranthema, allied genera, distant hybridization, dual-color genomic in situ hybridization

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