不同温度条件下常春藤叶片磷脂变化的比较分析

园艺学报 ›› 2003, Vol. 30 ›› Issue (4): 431-435.

不同温度条件下常春藤叶片磷脂变化的比较分析

沈漫

(北京农学院植物科学技术系，北京1o22o6)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-25 发布日期:2003-08-25

The Comparisons and the Analyses of Changes in Phospholipids from Leaf ofIvy Growing in Diferent Temperature Conditions

Shen Man

(Department ofPlant Science and Technology，8eiji~ Agriculture College，8eiji~ 102206，China)

Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

摘要/Abstract

摘要： 以栽培在露地和温室的常春藤(Hedera nepalensis,car．sinensis)为材料，运用荧光偏振、薄层层析扫描、气相色谱等技术测定叶片磷脂流动性、磷脂及其脂肪酸组成配比。结果表明，与温室栽培相比，地栽培的常春藤具有较高的不饱和磷脂和不饱和脂肪酸含量。在低温胁迫下，磷脂酰胆碱(PC)的不饱和脂肪酸水平是影响膜脂流动性的主要因素。通过对不同温度条件下生长的常春藤的磷脂流动性、磷脂和脂肪酸组成的差异性分析，从膜脂组成与结构的角度探讨了常绿阔叶植物的抗寒机理。

关键词: 常春藤, 磷脂, 脂肪酸, 膜脂流动性, 抗寒性

Abstract: Selected ivy(Hedera nepalensis vaur．sinensis)growing in open field and greenhouse separately as the experimental material，the fluidity an d the composition of fatty acids in phospholipids isolated from the leaves
were determined by fluorescence polarization，scanning thin—layer chroma tography and gas chroma tography methods．The results showed that ivy growing in ope n field in January possessed the higher level of unsaturated phospholipids (PC and PE)and fatty acids(16：1+18：1+18：2+18：3)than that growing in greenhouse． For ivy g∞ in open field，phosphatidyl—choline (PC )Was the main factor to influence its membralle—lipid fluidity during it
adapted to the low—temperature stress． By an alyzing the diferences of fluidity an d composition of phospholipids from the leaves of ivy growing in diffe~nt temperature conditions， the mechan ism of the cold—resistance of evergreen broad—leaved plant in terms of the composition and the structure of membrane—lipids were discussed．

Key words: Hedera nepalensis, Phospholipids, Fatty acids, Membrane—lipid fluidity, Cold—resistanc

中图分类号:

S 68

沈漫. 不同温度条件下常春藤叶片磷脂变化的比较分析[J]. 园艺学报, 2003, 30(4): 431-435.

Shen Man. The Comparisons and the Analyses of Changes in Phospholipids from Leaf ofIvy Growing in Diferent Temperature Conditions[J]. ACTA HORTICULTURAE SINICA, 2003, 30(4): 431-435.

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