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园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1344-1354.doi: 10.16420/j.issn.0513-353x.2016-0880

• 研究论文 • 上一篇    下一篇

弯刺蔷薇与大花白木香越冬抗寒性及其生理差异分析

刘 强,杨树华*,贾瑞冬,赵 鑫,葛 红*   

  1. 中国农业科学院蔬菜花卉研究所,国家花卉改良中心,北京 100081
  • 出版日期:2017-07-25 发布日期:2017-07-25

Analysis of Freezing Tolerances and Its Physiological Differences of Two Rosa Species During the Overwintering

LIU Qiang,YANG Shuhua*,JIA Ruidong,ZHAO Xin,and GE Hong*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,National Flowers Improvement Center of
    China,Beijing 100081,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

以弯刺蔷薇(Rosa beggeriana)和大花白木香(R. fortuneana)当年生叶片和枝条为试材,
采用电解质渗出率拟合Logistic 方程确定低温半致死温度(LT50)评价抗寒性,采用过碘酸雪夫氏染色观
察茎中淀粉积累,测定相对含水量、丙二醛、可溶性糖、脯氨酸、脱落酸含量以及超氧化物歧化酶、过
氧化物酶和过氧化氢酶活性,研究其越冬期间抗寒性变化及其生理基础。结果发现:两种蔷薇叶片抗寒
能力相当,但弯刺蔷薇枝条抗寒性远强于大花白木香。与大花白木香相比,弯刺蔷薇在低温驯化初期累
积了更多的可溶性糖,并在叶片脱落前可能通过增加的ABA 信号促进可溶性糖由叶片向茎中运输并贮存
为淀粉,在严冬时期淀粉水解形成更多的渗透调节物质,并通过更高的SOD 活性维持氧化还原平衡;而
大花白木香中只发现了比弯刺蔷薇中更高的脯氨酸含量和POD 活性,这可能难以维持其渗透压和氧化还
原平衡并造成了更多的膜脂过氧化伤害,最终导致其枝条的抗寒性远小于弯刺蔷薇。

关键词: 弯刺蔷薇, 大花白木香, 抗寒性, 越冬

Abstract:

To study the changes of freezing tolerance and its physiological basis of annual leaves and
shoots of Rosa beggeriana and R. fortuneana,the cold hardiness was estimated by the semi-lethal
temperature(LT50),which was assessed by electrolyte leakage rate with Logistic equation,the
accumulation of starches in stems was observed by periodic acid-shiff staining and the contents of relative
water,malondialdehyde,soluble sugars,proline,ABA and activities of SOD,POD and CAT were
measured. The results showed that the freezing tolerances of leaves of two Rosa species were similar,but
the shoots of R. beggeriana exhibited stronger cold hardiness than that of R. fortuneana. Compared with R.
fortuneana,R. beggeriana accumulated more soluble sugars at the beginning of cold acclimation,which
transported to shoots and stored as starch under the possible signaling regulation by increased ABA content before defoliation and hydrolyzed as soluble sugars at the midwinter to sustain osmotic balance,and
depended on higher activity of SOD to maintain redox balance,while the higher content of proline and
POD activity observed in the shoots of R. fortuneana could not sustain the osmotic and redox balance,
which led to more damage of membrane lipid peroxidation and weaker hardiness eventually.

Key words: Rosa beggeriana, R. fortuneana, freezing tolerance, overwintering